Category: Oocyte Handling

oocyte collection

Oocyte Collection Needle

Description / Use

The devices feature:

  • An echogenic triple bevel single lumen needle.
  • A thin tube connecting the needle to a bung.
  • A vacuum tube connecting the bung to the aspiration source.
  • A three-way flushing valve (for the model concerned), to choose between aspiration or rinsing the follicles.

The complete sets (needle + tubing) provide adapted storage conditions for the oocytes during the collection, particularly being able to maintain the vials at an appropriate temperature using heating systems. The resilient properties of polyurethane ensure flexibility without kinking for a regular flow rate, with no loss of suction.

Reference Description Packaging
1301020 OPS® Classic without valve Box of 10 units
1301021 OPS® Classic with valve Box of 10 units

ops

OPS

Description / Use

  • A single channel needle, triple-beveled, with a 5 mm grooved echogenic zone before the beveled tip.
  • A thin tubing connecting the needle to a bung. The latter is fitted onto a Falcon® type collecting tube.
  • A three-way valve (for the model concerned), to choose between aspiration or rinsing of the follicles.
  • A vacuum tube connecting the bung to the aspiration source.

The complete sets (needle + tubing) provide adapted storage conditions for the oocytes during the collection, particularly being able to maintain the vials at an appropriate temperature using heating systems. The resilient properties of polyurethane ensure flexibility without kinking for a regular flow rate, with no loss of suction. The specific quality of steel and polishing techniques improve the needle’s handling and sliding motion. The grooved circular markings provide echogenicity very close to the bevel.

Reference Description Packaging
1313101 OPS® ART of CCD® without valve Box of 10 units

vit kit thaw

Vit Kit®-Thaw

Description / Use

Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

4 Vials of TS (Thawing Solution, 4 x 2mL). Contains

  • 1 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of DS (Dilution Solution, 1 x 2mL). Contains

  • 0.5 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of WS (Washing Solution, 1 x 2mL). Contains

  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description
90137 TS, 4x1ml
90137 DS, 1x1ml
90137 WS, 1x2ml

vit kit freeze

Vit Kit®-Freeze

Description / Use

Vitrification Freeze Kit is for use in the vitrification of oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

2 Vials of ES (Equilibration Solution, 2 x 1 mL). Contains

  • 7.5% DMSO.
  • 7.5% ethylene glycol.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

2 Vials of VS (Vitrification Solution, 2 x 1 mL). Contains

  • 15% DMSO.
  • 15% ethylene glycol.
  • 20% DSS.
  • 0.5 M sucrose.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description Packaging
90133 ES, 1x1ml 10 Cryo Tips
90133 VS, 2x1ml 10 Cryo Tips

hsv straw red

HSV High Security Vitrification Straw – Red

Description / Use

High Security Vitrification (HSV) Red Straw (4 straws)

The only high security closed system for oocyte & embryo vitrification HSV straws provide the highest quality cryopreservation of biological samples.

Quality Control Testing:

  • Advanced Vitrification Techniques.
  • Vitrification and Warming of Blastocysts.
  • Pregnancy results following initiation of a blastocyst vitrification program.
  • Vitrification and warming of donor oocytes using a closed straw system.
Reference Description Packaging
025246 HSV High Security Vitrification kits – Red Pack of 4
025247 HSV High Security Vitrification kits – White Pack of 4
025248 HSV High Security Vitrification kits – Yellow Pack of 4
025249 HSV High Security Vitrification kits – Purple Pack of 4
025250 HSV High Security Vitrification kits – Blue Pack of 4
025251 HSV High Security Vitrification kits – Green Pack of 4

hyaluronidase solution

Hyaluronidase Solution

Description / Use

Hyaluronidase Solution is a convenient, ready-to-use solution designed for removing cumulus cells surrounding freshly retrieved oocytes prior to ICSI or other ART procedures.

This solution consists of 80 IU/mL of Hyaluronidase enzyme in a HEPES buffered HTF medium with therapeutic-grade human serum albumin (50mb/m:) and the antibiotic gentamicin sulfate (10µg/mL).

Irvine Scientific has performed extensive efficacy testing on Hyaluronidase Solution using freshly retrieved mouse oocytes and one-cell embryos.

The performance of Irvine Scientific’s Hyaluronidase Solution was equivalent to control hyaluronidase solutions, and no toxicity was observed to mouse oocytes and embryos. The development of one-cell embryos to blastocysts was equivalent after exposure to Hyaluronidase Solution as compared to non exposed controls (in growth medium only). The lot-to-lot performance of HJyaluronidase Solution was consistent for enzyme activity and the removal of cumulus cells from freshly retrieved oocytes with in 2 minutes.

In addition to the mouse oocyte and embryo bioassays, Hyaluronidase Solution is also tested for enzyme activity using a standard enzymatic assay for Hyaluronidase. This permits verification of actual enzyme activity, in addition to the functional bioassay of cumulus dell removal and lack of toxicity.

Morover, stability studies have been conducted to determine the enzymatic activity of Irvine Scientific’s Hyaluronidase Solution after storage at several temperatures. The results is greater than 95% of the initial enzymatic activity (80IU/mL) remains after storage for 2 months at 6℃ (refrigerated) or 6 months frozen (-10℃).

The recommended storage temperature for this product is frozen (<-10℃) to assure a shelf life of at least 6 months. Repeated freeze/thaw cycles of Hyaluronidase Solution (no more than 3) did not adversely affect the enzymatic activity.

Hyaluronidase Solution

  • Available in kits of 5×1.0mL.
  • The shelf life is 6 months when stored sealed at the recommended temperature of (<-10℃).
  • Gentamicin sulfate is included as the antibiotic.

Features And Benefits

  • Convenient ready-to-use solution for denuding oocytes of cumulus cells prior to ICSI or other ART procedures. Refer to product insert for detailed usage instructions.
  • This solution contains HEPES buffered HTF medium to maintain proper pH during cumulus cell removal at ambient atmosphere conditions.
Reference Packaging
90101 5x1mL

modified htf medium

Modified HTF Medium with Gentamicin – HEPES

Description / Use

Modified HTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos. mHTF is composed of a HEPES and sodium bicarbonate buffering system, making it ideal for use in ambient atmosphere conditions. It requires protein supplementation before use.

Quality Control Testing:

  • Store at 2-8℃.
  • 12 months from date of manufacture.
Reference Description
90166 100ml
90166 500ml

mhm c with gentamicin

Multipurpose Handling Medium® -Complete (MHM®-C) with Gentamicin

Description / Use

Multipurpose Handling Medium® (MHM®) is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

  • Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
  • Support maintenance of cellular homeostasis with aid of glycine and taurine.
  • Reduce risk of toxicity using a dual buffer system.
  • Save time – no mixing or filtering, ready to use.
Reference Description
99166 100ml
99166 500ml

MHM With Gentamicin

Multipurpose Handling Medium® (MHM®) with Gentamicin

 

Description / Use

Multipurpose Handling Medium® (MHM®) with Gentamicin is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

  • Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
  • Support maintenance of cellular homeostasis with aid of glycine and taurine.
  • Reduce risk of toxicity using a dual buffer system.
  • Add preferred protein supplements as needed.
Reference Description
99166 100ml
99166 500ml