Cryo Bio System

lyophilized

Polyvinylpyrrolidone (PVP) – Lyophilized

Description / Use

Polyvinylpyrrolidone (PVP) – Lyophilized is intended for use in assisted reproductive procedures that include gamete and embryo manipulation. These procedures include the use of PVP solution for immobilizing sperm for ICSI procedures. PVP – Lyophilized is a powder form which requires aseptic reconstitution with mHTF or other appropriate media prior to use.

As a powder, PVP – Lyophilized has a 2 year shelf life and allows for custom PVP concentrations to suit your laboratory processes. Once reconstituted, lyophilized PVP may be stored for up to 3 weeks. This product does not contain protein supplement.

Quality Control Testing:

Endotoxin by Limulus Amebocyte Lysate (LAL).
Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
Sperm Motility Recovery Assay.
Store at 2-8℃
Shelf Life 2 years from date of manufacture.

Reference	Description
90121	5×0.5mL (7%)
90123	5×0.5mL (10%)

solution with hsa 10

Polyvinylpyrrolidone (PVP) Solution with HSA – 10%

Description / Use

Polyvinylpyrrolidone (PVP) 10% is a viscous solution that allows for easier sperm micromanipulation by slowing or decreasing the motility of sperm for intracytoplasmic sperm injection (ICSI). PVP 10% has relatively more viscosity than PVP 7% and is recommended for use with high motility and healthy sperm. PVP solution yields greater control over fluid flow and prevents sticking in the ICSI pipette.

Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
CE Marked.

Quality Control Testing:

Endotoxin by Limulus Amebocyte Lysate (LAL).
Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
perm Motility Recovery Assay.
Store at 2-8℃.
Shelf Life 6 months from date of manufacture.

Reference	Description
90121	5×0.5mL (7%)
90123	5×0.5mL (10%)

solution with hsa 7

Polyvinylpyrrolidone (PVP) Solution with HSA – 7%

Description / Use

Polyvinylpyrrolidone (PVP) with HSA – 7% is a viscous solution that allows for easier sperm micromanipulation by slowing or immobilizing sperm for intracytoplasmic sperm injection (ICSI). PVP 7% has relatively less viscosity than PVP 10% and is recommended for use with low quality or low motility sperm. PVP solution also yields greater control over fluid flow and prevents sticking in the ICSI pipette.

Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
CE Marked.

Endotoxin by Limulus Amebocyte Lysate (LAL).
Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
Sperm Motility Recovery Assay.
Store at 2-8℃.
Shelf Life 6 months from date of manufacture.

Reference	Description
90121	5×0.5mL (7%)
90123	5×0.5mL (10%)

hyaluronidase solution

Hyaluronidase Solution

Description / Use

Hyaluronidase Solution is a convenient, ready-to-use solution designed for removing cumulus cells surrounding freshly retrieved oocytes prior to ICSI or other ART procedures.

This solution consists of 80 IU/mL of Hyaluronidase enzyme in a HEPES buffered HTF medium with therapeutic-grade human serum albumin (50mb/m:) and the antibiotic gentamicin sulfate (10µg/mL).

Irvine Scientific has performed extensive efficacy testing on Hyaluronidase Solution using freshly retrieved mouse oocytes and one-cell embryos.

The performance of Irvine Scientific’s Hyaluronidase Solution was equivalent to control hyaluronidase solutions, and no toxicity was observed to mouse oocytes and embryos. The development of one-cell embryos to blastocysts was equivalent after exposure to Hyaluronidase Solution as compared to non exposed controls (in growth medium only). The lot-to-lot performance of HJyaluronidase Solution was consistent for enzyme activity and the removal of cumulus cells from freshly retrieved oocytes with in 2 minutes.

In addition to the mouse oocyte and embryo bioassays, Hyaluronidase Solution is also tested for enzyme activity using a standard enzymatic assay for Hyaluronidase. This permits verification of actual enzyme activity, in addition to the functional bioassay of cumulus dell removal and lack of toxicity.

Morover, stability studies have been conducted to determine the enzymatic activity of Irvine Scientific’s Hyaluronidase Solution after storage at several temperatures. The results is greater than 95% of the initial enzymatic activity (80IU/mL) remains after storage for 2 months at 6℃ (refrigerated) or 6 months frozen (-10℃).

The recommended storage temperature for this product is frozen (<-10℃) to assure a shelf life of at least 6 months. Repeated freeze/thaw cycles of Hyaluronidase Solution (no more than 3) did not adversely affect the enzymatic activity.

Hyaluronidase Solution

Available in kits of 5×1.0mL.
The shelf life is 6 months when stored sealed at the recommended temperature of (<-10℃).
Gentamicin sulfate is included as the antibiotic.

Features And Benefits

Convenient ready-to-use solution for denuding oocytes of cumulus cells prior to ICSI or other ART procedures. Refer to product insert for detailed usage instructions.
This solution contains HEPES buffered HTF medium to maintain proper pH during cumulus cell removal at ambient atmosphere conditions.

Reference	Packaging
90101	5x1mL

mhm c with gentamicin

Multipurpose Handling Medium® -Complete (MHM®-C) with Gentamicin

Description / Use

Multipurpose Handling Medium® (MHM®) is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
Support maintenance of cellular homeostasis with aid of glycine and taurine.
Reduce risk of toxicity using a dual buffer system.
Save time – no mixing or filtering, ready to use.

Reference	Description
99166	100ml
99166	500ml

MHM With Gentamicin

Multipurpose Handling Medium® (MHM®) with Gentamicin

Description / Use

Multipurpose Handling Medium® (MHM®) with Gentamicin is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
Support maintenance of cellular homeostasis with aid of glycine and taurine.
Reduce risk of toxicity using a dual buffer system.
Add preferred protein supplements as needed.

Reference	Description
99166	100ml
99166	500ml