Cryo Bio System

lyophilized

Polyvinylpyrrolidone (PVP) – Lyophilized

Description / Use

Polyvinylpyrrolidone (PVP) – Lyophilized is intended for use in assisted reproductive procedures that include gamete and embryo manipulation. These procedures include the use of PVP solution for immobilizing sperm for ICSI procedures. PVP – Lyophilized is a powder form which requires aseptic reconstitution with mHTF or other appropriate media prior to use.

As a powder, PVP – Lyophilized has a 2 year shelf life and allows for custom PVP concentrations to suit your laboratory processes. Once reconstituted, lyophilized PVP may be stored for up to 3 weeks. This product does not contain protein supplement.

Quality Control Testing:

Endotoxin by Limulus Amebocyte Lysate (LAL).
Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
Sperm Motility Recovery Assay.
Store at 2-8℃
Shelf Life 2 years from date of manufacture.

Reference	Description
90121	5×0.5mL (7%)
90123	5×0.5mL (10%)

solution with hsa 10

Polyvinylpyrrolidone (PVP) Solution with HSA – 10%

Description / Use

Polyvinylpyrrolidone (PVP) 10% is a viscous solution that allows for easier sperm micromanipulation by slowing or decreasing the motility of sperm for intracytoplasmic sperm injection (ICSI). PVP 10% has relatively more viscosity than PVP 7% and is recommended for use with high motility and healthy sperm. PVP solution yields greater control over fluid flow and prevents sticking in the ICSI pipette.

Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
CE Marked.

Quality Control Testing:

Endotoxin by Limulus Amebocyte Lysate (LAL).
Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
perm Motility Recovery Assay.
Store at 2-8℃.
Shelf Life 6 months from date of manufacture.

Reference	Description
90121	5×0.5mL (7%)
90123	5×0.5mL (10%)

solution with hsa 7

Polyvinylpyrrolidone (PVP) Solution with HSA – 7%

Description / Use

Polyvinylpyrrolidone (PVP) with HSA – 7% is a viscous solution that allows for easier sperm micromanipulation by slowing or immobilizing sperm for intracytoplasmic sperm injection (ICSI). PVP 7% has relatively less viscosity than PVP 10% and is recommended for use with low quality or low motility sperm. PVP solution also yields greater control over fluid flow and prevents sticking in the ICSI pipette.

Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
CE Marked.

Endotoxin by Limulus Amebocyte Lysate (LAL).
Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
Sperm Motility Recovery Assay.
Store at 2-8℃.
Shelf Life 6 months from date of manufacture.

Reference	Description
90121	5×0.5mL (7%)
90123	5×0.5mL (10%)

htf medium gentamicin

HTF Medium with Gentamicin

Description / Use

HTF Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation through Day 3 of development. HTF Medium is a synthetic, defined solution for use as a culture media through Day 3 of human embryo development as well as the processing of gametes. HTF is bicarbonate-based and is designed for use in a CO2 incubator.

Requires protein supplementation 100 mL.

Note : 500 mL packaging available by standing order.

Quality Control Testing:

Store at 2-8℃.
Shelf Life 64 days from date of manufacture.

Reference	Description
90125	100mL
90125	500mL
90126	100mL
90126	500mL

medium gentamicin

P-1® Medium with Gentamicin

Description / Use

P-1® Medium is intended for use in culturing human gametes and embryos during fertilization and growth through Day 3 of development. P-1® Medium was developed by Dr. Thomas B. Pool, Fertility Center of San Antonio, based upon the original HTF formula but modified to provide a glucose-free and phosphate-free environment. This formulation also contains taurine, an amino acid shown to have protective and embryogenic functions. P-1® Medium is bicarbonate-based and is designed for procedures utilizing a CO2 incubator.

Requires Protein Supplementation. 100 mL.

Quality Control Testing:

Store at 2-8℃.
Shelf Life 64 days from date of manufacture.

Reference	Description
90125	100mL
90125	500mL
90126	100mL
90126	500mL

continuous single culture hsa

Continuous Single Culture® Complete with HSA

Description / Use

Continuous Single Culture® Complete product (CSCM-C) with Human Serum Albumin is a novel solution designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system without dish change or medium renewal.

Pre-supplemented with HSA for a final total protein concentration of 5 mg/mL.

Quality Control Testing:

Available in 2x20mL configuration.
Store at 2-8℃.

Reference	Description
90165	2x20ml CSC Complete

continuous single culture

Continuous Single Culture

Description / Use

Culture media for human gametes and embryos during fertilization and growth of embryos through Day 5/6 of development.

Continuous Single Culture

Available in 60 mL configuration.
Shelf life is 90 days from date of manufacture.
Shelf life after opening is 8 weeks.
Contains Gentamicin as the antibiotic.
Requires protein supplementation.

Continuous Single Culture Complete

Available in 2×20 mL Kits.
Shelf life is 120 days from date of manufacture.
Ready to use, pre-supplemented with Human Serum Albumin (10% v/v HSA), for a final total protein concentration of 5mg/mL.

Features And Benefits

Continuous Single Culture Media is the only true continuous culture medium that is clinically proven to improve implantation rates over a sequential media system.

For use from fertilization through Day 5/6 of development.
Optimized for use in a continuous culture system, without the need to refresh medium.
Only one medium to order, inventory and store.

Reference	Description	Packaging
90165	CSC Complete	2×20 ml

continuous single culture nx

Continuous Single Culture®- NX

Description / Use

Continuous Single Culture® – NX (CSCM-NX) is a clinically-proven, low lactate, single-step culture medium that helps improves blastocyst development. CSCM-NXC is designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system, without dish change or medium renewal.

Quality Control Testing:

Storage : 2-8℃.
Shelf Life : 120 days
Gentamicin.
In a M-199 HEPES Buffered Medium.

Reference	Description
90165	2x20ml CSC Complete

hyaluronidase solution

Hyaluronidase Solution

Description / Use

Hyaluronidase Solution is a convenient, ready-to-use solution designed for removing cumulus cells surrounding freshly retrieved oocytes prior to ICSI or other ART procedures.

This solution consists of 80 IU/mL of Hyaluronidase enzyme in a HEPES buffered HTF medium with therapeutic-grade human serum albumin (50mb/m:) and the antibiotic gentamicin sulfate (10µg/mL).

Irvine Scientific has performed extensive efficacy testing on Hyaluronidase Solution using freshly retrieved mouse oocytes and one-cell embryos.

The performance of Irvine Scientific’s Hyaluronidase Solution was equivalent to control hyaluronidase solutions, and no toxicity was observed to mouse oocytes and embryos. The development of one-cell embryos to blastocysts was equivalent after exposure to Hyaluronidase Solution as compared to non exposed controls (in growth medium only). The lot-to-lot performance of HJyaluronidase Solution was consistent for enzyme activity and the removal of cumulus cells from freshly retrieved oocytes with in 2 minutes.

In addition to the mouse oocyte and embryo bioassays, Hyaluronidase Solution is also tested for enzyme activity using a standard enzymatic assay for Hyaluronidase. This permits verification of actual enzyme activity, in addition to the functional bioassay of cumulus dell removal and lack of toxicity.

Morover, stability studies have been conducted to determine the enzymatic activity of Irvine Scientific’s Hyaluronidase Solution after storage at several temperatures. The results is greater than 95% of the initial enzymatic activity (80IU/mL) remains after storage for 2 months at 6℃ (refrigerated) or 6 months frozen (-10℃).

The recommended storage temperature for this product is frozen (<-10℃) to assure a shelf life of at least 6 months. Repeated freeze/thaw cycles of Hyaluronidase Solution (no more than 3) did not adversely affect the enzymatic activity.

Hyaluronidase Solution

Available in kits of 5×1.0mL.
The shelf life is 6 months when stored sealed at the recommended temperature of (<-10℃).
Gentamicin sulfate is included as the antibiotic.

Features And Benefits

Convenient ready-to-use solution for denuding oocytes of cumulus cells prior to ICSI or other ART procedures. Refer to product insert for detailed usage instructions.
This solution contains HEPES buffered HTF medium to maintain proper pH during cumulus cell removal at ambient atmosphere conditions.

Reference	Packaging
90101	5x1mL

mhm c with gentamicin

Multipurpose Handling Medium® -Complete (MHM®-C) with Gentamicin

Description / Use

Multipurpose Handling Medium® (MHM®) is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
Support maintenance of cellular homeostasis with aid of glycine and taurine.
Reduce risk of toxicity using a dual buffer system.
Save time – no mixing or filtering, ready to use.

Reference	Description
99166	100ml
99166	500ml