Category: Cryopreservation day 3-5

sperm maintenance medium

Sperm Maintenance Medium

Description / Use

Sperm Maintenance Medium uses only 1 mL of medium per 3 mL of semen or sperm. This more efficient medium means lower costs per cycle of sperm cryopreservation, minimal dilution of low quality semen, and potentially fewer straws/vials per patient. Sperm Maintenance Medium has similar performance to Freezing Medium TYB, a TEST-yolk buffer based sperm cryopreservation medium, without containing egg yolk (Es-slami, 2009). The medium’s unique formulation contains ingredients, such as hypotaurine and ascorbic acid, that have been shown to protect against reactive oxygen species.

  • CE Marked.
  • Contains:
    – 28% v/v glycerol for a 3:1 ratio of semen to medium
    – Antioxidants, such as hypotaurine and ascorbic acid
    – HEPES
    – Human Serum Albumin
    – Sucrose
    – Pluronic F-68.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Sperm Cryosurvival assay.
  • Sterility by the current USP , CFR or Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
  • Store below -10℃
  • Shelf life Product should be used within (8) weeks from opening when stored under the recommended conditions of 2 to 8℃.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refirigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media

vit kit thaw

Vit Kit®-Thaw

Description / Use

Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

4 Vials of TS (Thawing Solution, 4 x 2mL). Contains

  • 1 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of DS (Dilution Solution, 1 x 2mL). Contains

  • 0.5 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of WS (Washing Solution, 1 x 2mL). Contains

  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description
90137 TS, 4x1ml
90137 DS, 1x1ml
90137 WS, 1x2ml

vit kit freeze

Vit Kit®-Freeze

Description / Use

Vitrification Freeze Kit is for use in the vitrification of oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

2 Vials of ES (Equilibration Solution, 2 x 1 mL). Contains

  • 7.5% DMSO.
  • 7.5% ethylene glycol.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

2 Vials of VS (Vitrification Solution, 2 x 1 mL). Contains

  • 15% DMSO.
  • 15% ethylene glycol.
  • 20% DSS.
  • 0.5 M sucrose.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description Packaging
90133 ES, 1x1ml 10 Cryo Tips
90133 VS, 2x1ml 10 Cryo Tips

hsv straw red

HSV High Security Vitrification Straw – Red

Description / Use

High Security Vitrification (HSV) Red Straw (4 straws)

The only high security closed system for oocyte & embryo vitrification HSV straws provide the highest quality cryopreservation of biological samples.

Quality Control Testing:

  • Advanced Vitrification Techniques.
  • Vitrification and Warming of Blastocysts.
  • Pregnancy results following initiation of a blastocyst vitrification program.
  • Vitrification and warming of donor oocytes using a closed straw system.
Reference Description Packaging
025246 HSV High Security Vitrification kits – Red Pack of 4
025247 HSV High Security Vitrification kits – White Pack of 4
025248 HSV High Security Vitrification kits – Yellow Pack of 4
025249 HSV High Security Vitrification kits – Purple Pack of 4
025250 HSV High Security Vitrification kits – Blue Pack of 4
025251 HSV High Security Vitrification kits – Green Pack of 4

freezing medium glycerol

Freezing Medium – TYB with Glycerol & Gentamicin

Description / Use

Freezing Medium TYB contains TEST-yolk buffer (TYB) to recover more motile sperm post-thaw than glycerol-only media (Hallak 2000, Nallella 2004). TEST-yolk buffer is a semen extender and cryobuffer that can be paired with glycerol to achieve high sperm motility and viability. The egg yolk is from USDA certified virus-free flocks and washed from the sperm after cryopreservation. Freezing Medium TYB is also the only CE-marked and FDA-approved sperm freezing media containing TYB and glycerol. As one of the most referenced sperm cryopreservation media worldwide, Freezing Medium TYB has established itself as the standard for performance.

  • 2 year shelf life.
  • Contains:
    – 20% egg yolk from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56&#8451 for 30 minutes.
    – 12% glycerol.
    – 10µg/mL gentamicin sulfate.
  • CE Marked.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Sperm Cryosurvival assay.
  • Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refirigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media