Category: Embryo Culture

sperm maintenance medium

Sperm Maintenance Medium

Description / Use

Sperm Maintenance Medium uses only 1 mL of medium per 3 mL of semen or sperm. This more efficient medium means lower costs per cycle of sperm cryopreservation, minimal dilution of low quality semen, and potentially fewer straws/vials per patient. Sperm Maintenance Medium has similar performance to Freezing Medium TYB, a TEST-yolk buffer based sperm cryopreservation medium, without containing egg yolk (Es-slami, 2009). The medium’s unique formulation contains ingredients, such as hypotaurine and ascorbic acid, that have been shown to protect against reactive oxygen species.

  • CE Marked.
  • Contains:
    – 28% v/v glycerol for a 3:1 ratio of semen to medium
    – Antioxidants, such as hypotaurine and ascorbic acid
    – HEPES
    – Human Serum Albumin
    – Sucrose
    – Pluronic F-68.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Sperm Cryosurvival assay.
  • Sterility by the current USP , CFR or Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
  • Store below -10℃
  • Shelf life Product should be used within (8) weeks from opening when stored under the recommended conditions of 2 to 8℃.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refirigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media

embryo biopsy medium

Embryo Biopsy Medium

Description / Use

Embryo Biopsy Medium is for temporary culture and holding of day 3 embryos during biopsy. It is an ideal environment for 4-10 cell embryos that will have blastomeres removed for pre-implantation genetic screening or diagnosis.

Contains:

  • HEPES-buffered HTF without Ca2+ and Mg2+.
  • EDTA as chelating agent.
  • Human serum albumin (5 mg/mL).
  • Gentamicin sulfate (10µg/mL).

Quality Control Testing:

  • Store at 2-8℃
  • Shelf Life 180 days from date of manufacture.
Reference Description
90116 6x10ml
90124 6x10ml

ecm with dss

Complete Early Cleavage Medium® (ECM®) with DSS

Description / Use

Complete Early Cleavage Medium® (ECM®) is intended for use in culturing human gametes during fertilization (IVF) and growth of embryos through Day 3 of development. ECM® is a modification of P-1® Medium that contains glucose, alanyl-glutamine and EDTA, components shown to benefit human embryo development. ECM® is bicarbonate based and is designed for procedures utilizing a CO2 incubator.

Available in 2 x 20 mL configuration. Already supplemented with protein (10% DSS) for a final total macromolecule concentration of 7 mg/mL.

Quality Control Testing:

  • Store at 2-8℃
  • Shelf Life 120 days from date of manufacture.

continuous single culture hsa

Continuous Single Culture® Complete with HSA

Description / Use

Continuous Single Culture® Complete product (CSCM-C) with Human Serum Albumin is a novel solution designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system without dish change or medium renewal.

Pre-supplemented with HSA for a final total protein concentration of 5 mg/mL.

Quality Control Testing:

  • Available in 2x20mL configuration.
  • Store at 2-8℃.
Reference Description
90165 2x20ml CSC Complete

continuous single culture

Continuous Single Culture

Description / Use

Culture media for human gametes and embryos during fertilization and growth of embryos through Day 5/6 of development.

Continuous Single Culture

  • Available in 60 mL configuration.
  • Shelf life is 90 days from date of manufacture.
  • Shelf life after opening is 8 weeks.
  • Contains Gentamicin as the antibiotic.
  • Requires protein supplementation.

Continuous Single Culture Complete

  • Available in 2×20 mL Kits.
  • Shelf life is 120 days from date of manufacture.
  • Ready to use, pre-supplemented with Human Serum Albumin (10% v/v HSA), for a final total protein concentration of 5mg/mL.

Features And Benefits

    Continuous Single Culture Media is the only true continuous culture medium that is clinically proven to improve implantation rates over a sequential media system.

  • For use from fertilization through Day 5/6 of development.
  • Optimized for use in a continuous culture system, without the need to refresh medium.
  • Only one medium to order, inventory and store.
Reference Description Packaging
90165 CSC Complete 2×20 ml

continuous single culture nx

Continuous Single Culture®- NX

Description / Use

Continuous Single Culture® – NX (CSCM-NX) is a clinically-proven, low lactate, single-step culture medium that helps improves blastocyst development. CSCM-NXC is designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system, without dish change or medium renewal.

Quality Control Testing:

  • Storage : 2-8℃.
  • Shelf Life : 120 days
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description
90165 2x20ml CSC Complete

vit kit thaw

Vit Kit®-Thaw

Description / Use

Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

4 Vials of TS (Thawing Solution, 4 x 2mL). Contains

  • 1 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of DS (Dilution Solution, 1 x 2mL). Contains

  • 0.5 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of WS (Washing Solution, 1 x 2mL). Contains

  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description
90137 TS, 4x1ml
90137 DS, 1x1ml
90137 WS, 1x2ml

vit kit freeze

Vit Kit®-Freeze

Description / Use

Vitrification Freeze Kit is for use in the vitrification of oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

2 Vials of ES (Equilibration Solution, 2 x 1 mL). Contains

  • 7.5% DMSO.
  • 7.5% ethylene glycol.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

2 Vials of VS (Vitrification Solution, 2 x 1 mL). Contains

  • 15% DMSO.
  • 15% ethylene glycol.
  • 20% DSS.
  • 0.5 M sucrose.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description Packaging
90133 ES, 1x1ml 10 Cryo Tips
90133 VS, 2x1ml 10 Cryo Tips