Category: Vitrification Freeze and Thaw Media

Description / Use

The SYMS III sealer is a bench top unit that can be used under laminar rlow hood and is using the “thermal” procedure to weld CBS High Security 0.3mL, 0.5mL and 2mL straws, CBS High Security Tubes as well as the HSV High Security Vitrification Straw.

Technical Specifications

Setup, maintenance and downtime time are approximately 5 minutes per day.

Potentially contaminated parts of the system can be cleaned with ethanol or non-corrosive decontamination fluids.

L x W x H: 363 x 276 x 152mm
Weight: 6kg
Power Supply: 110 or 220V/60 or 50Hz

Key Features

• Available in 3 languages (French, English or Spanish).
• Glove compatible touch screen interface.
• Easy-to-use touch screen advises user of the machines actions:
  heating up, ready, welding process, etc.
• Pre-set specific combination of seal temperature, time and jaw position for each CBS device.
• Two sealing modes: fully automatic straw detection system or touch screen operator control.
• Error management: alarm message on touch screen and audible signal.
• Electronic touch screen monitors and advises on the maintenance needs of the machine.
• Easy to clean and maintain.

Instructions For Use

• Place the CBS High Security straw of the CBS High Security tube holder;
• Place the straw or tube on the holder and gently push forward;
• Automatic mode: As soon as the sensor detects the straw or tube in the sealing position, the sealing process starts automatically.
• Manual mode: the straw or tube is sealed when the operator presses the ‘WELD’ button on touch screen.

Description / Use

Cryo Bio System carries a line of accessories for storage and management of HSV kits in liquid nitrogen.

Thawing

Thawing of the specimen should be done in a controlled environment, such as a laminar flow hood.

• Prepare the thawing/dilution media
• Identify the HSV straw
• Transfer the straw from the storage container to a transport dewar filled with liquid nitrogen.
• Lift the straw enough to expose the colored handling rod. Make sure the end with the sample remains immersed in the liquid nitrogen.
• While holding the straw, use the opening device for HSV kit to section the straw.
• Immediately (within 2 seconds), plunge the gutter into the first dilution media.

Shelf-Life

• Three years from date of manufacture.

Description / Use

Embryo Freeze and Thaw Media kits consist of convenient, ready-to-use solutions designed for the cryopreservation and recovery of cleavage-stage embryos (zygote to 8-cell).

The composition of these solutions is based upon published standard propanediol and sucrose frezing (2 step) and thawing (3 step) protocols, which have proven to be the most clinically successful methods for cryopreservation and recovery of zygote to early cleavage stage human embryos, achieving similar implantation and pregnancy rates to that for transfers of fresh embryos.

Irvine Scientific has performed extensive efficacy testing on our Embryo Freeze and Thaw Media using mouse embryos and two different bioassay methods. Since standard MEA (exposure only) does not specifically emulate the intended application of these products; and as part of our ongoing efforts to enhance the sensitivity of our QC test methods, a specific Embryo Recovery Assay was developed and used to emulate the intended use of these products.

These solutions were also evaluated using the standard one-cell mouse embryo assay, typically employed as the accepted QC text method for commercially available formulas.

Independent IVF laboratories showed that the recovery of one-cell cryopreserved mouseembryos from Irvine Scientific’s Embryo Freeze and Thaw Media was equivalent to, or better than that achieved for their control cryopreservation media (in house prepared media using either propanediol / sucrose or DMSI methods) currently used in clinical practice for cryopreservation of human embryos.

Use of the embryo Recovery Assay (with one-cell mouse embryos) is a highly sensitive performance test for the intended use of Irvine Scientific’s Embryo Freeze and Thaw Media. High blastocyst development rates were obtained using the standard mouse embryo assay. Which confirm the lack of toxicity of these solutions. The use of these media can achieve up to 100% recovery of cryopreserved cleavage-stage embryos.

Embryo Freeze Media Kit

• Embryo Freeze Medium-F1,2x10mL
• Embryo Freeze Medium-F1,4x10mL

Embryo Thaw Media Kit

• Embryo Thaw Medium-T1,2x10mL
• Embryo Thaw Medium-T2,2x10mL
• Embryo Thaw Medium-T2,2x10mL

Features And Benefits

• Convenient ready-to-use kits consisting of two media for cryopreservation and three media for the thawing of embryos.
• Rapid protocol with just two steps to freeze and thee steps to thaw.
• Each solution contains HTF medium buffered with HEPES to maintain proper pH during cryopreservation and thawing procedures at ambient temperature and atmosphere.
• Gentamicin sulfate is included in each medium as an antibiotic.
• Store at 2-8℃.
• Shelf life of 6 months.

Description / Use

Irvine Scientific’s Vit Kit® – Freeze and Vit Kit® – Thaw consist of solutions optimized for vitrification and recovery of oocytes (MII stage)
and embryos (zygote to blastocyst stage).

Media Composition

The Composition of these solutions is based upon publish methods utilizing a combination of ethylene glycol (EG) and dimethylsulfoxide (DMSO) as the permeating cryoprotectants. The basal medium for these solutions consists of Modified M199 (containing 21mM HEPES buffer) supplemented with 20% DSS(HSA and Dextran, containing 10 mg/mL protein) and 35ug/mL gentamicin as the antibiotic.

Advantages of Irvine Scientific’s Vitrification system

• Proven formulation that can be used for oocytes and all stages of embryos.
• Kits provide multiple applications and can be used on more than one patient.
• 8 weeks shelf-life after opening.
• The solutions are HEPES buffered to maintain pH during procedures performed at room temperature.
• Simple, straight forward protocols.
• Convenient configurations available with the CryoTip®, HSV straw, or with just the solutions.
• CryoTip® is an FDA approved closed freezing device.