Category: ART Culture Media

human tubal fluid

Human Tubal Fluid

Description / Use

HTF Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation through Day 3 of development

HTF Medium is a synthetic, defined solution for use as a culture media through Day 3 of human embryo development as well as the processing of gametes. HTF is bicarbonate-based and is designed for use in a CO2 incubator.

HTF Medium

  • Available as liquid medium in 100mL configuration. Custom configurations are available.
  • Has a shelf life of 64 days from the date of manufacture.
  • Requires protein supplementation.

Complete HTF Medium with SSS

  • Available as liquid medium in 2x20mL configuration.
  • Has a shelf life of 120 days from the date of manufacture.
  • Ready to use, already supplemented with protein (10% SSS) for a final total protein concentration of 6 mg/mL.

    • mHTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos.
    mHTF uses a buffering system composed of a 21 mM HEPES and 4 mM Sodium Bicarbonate combination. This buffering system does not require the use of a CO2 incubator.

mHTF Medium

  • Available as liquid medium in 100 mL configuration. Custom configurations are available.
  • Has a shelf life of 180 days from the date of manufacture.
  • Requires protein supplementation.
Reference Description Packaging
90125 HTF Media 100 ml
90125 HTF Media 500 ml
90126 mHTF 100 ml
90126 mHTF 500 ml

cbs vitrification straw

CBS High Security Vitrification Straw

Description / Use

Vitrification of embryos.
This product consists of three distinct parts:

  • A High Security ionomeric resin straw
  • A capillary tube with a pre-formed gutter co-injected with its colored handling rod
  • A blue plastic insertion device.

Sterilized by irradiation.
Packaged in a peel off blister pack (4 kits).

Identification

The HSV straw can be labeled using LN2 resistant labels.

Storage In Liquid Nitrogen

Cryo Bio System carries a line of accessories for storage and management of CBS High Security vitrification straws in liquid nitrogen (visotubes, goblets, canisters).

Instruction For Use

Reference Description Packaging
025246 CBS High Security Vitrification straw -Red Batch of 4
025247 CBS High Security Vitrification straw -White Batch of 4
025248 CBS High Security Vitrification straw -Yellow Batch of 4
025249 CBS High Security Vitrification straw -Pink Batch of 4
025250 CBS High Security Vitrification straw -Blue Batch of 4
025251 CBS High Security Vitrification straw -Green Batch of 4
022406 Opening device for CBS High Security Vitrification Straw 1

continuous single culture

Continuous Single Culture

Description / Use

Culture media for human gametes and embryos during fertilization and growth of embryos through Day 5/6 of development.

Continuous Single Culture

  • Available in 60 mL configuration.
  • Shelf life is 90 days from date of manufacture.
  • Shelf life after opening is 8 weeks.
  • Contains Gentamicin as the antibiotic.
  • Requires protein supplementation.

Continuous Single Culture Complete

  • Available in 2×20 mL Kits.
  • Shelf life is 120 days from date of manufacture.
  • Ready to use, pre-supplemented with Human Serum Albumin (10% v/v HSA), for a final total protein concentration of 5mg/mL.

Features And Benefits

    Continuous Single Culture Media is the only true continuous culture medium that is clinically proven to improve implantation rates over a sequential media system.

  • For use from fertilization through Day 5/6 of development.
  • Optimized for use in a continuous culture system, without the need to refresh medium.
  • Only one medium to order, inventory and store.
Reference Description Packaging
90165 CSC Complete 2×20 ml

hyaluronidase solution

Hyaluronidase Solution

Description / Use

Hyaluronidase Solution is a convenient, ready-to-use solution designed for removing cumulus cells surrounding freshly retrieved oocytes prior to ICSI or other ART procedures.

This solution consists of 80 IU/mL of Hyaluronidase enzyme in a HEPES buffered HTF medium with therapeutic-grade human serum albumin (50mb/m:) and the antibiotic gentamicin sulfate (10µg/mL).

Irvine Scientific has performed extensive efficacy testing on Hyaluronidase Solution using freshly retrieved mouse oocytes and one-cell embryos.

The performance of Irvine Scientific’s Hyaluronidase Solution was equivalent to control hyaluronidase solutions, and no toxicity was observed to mouse oocytes and embryos. The development of one-cell embryos to blastocysts was equivalent after exposure to Hyaluronidase Solution as compared to non exposed controls (in growth medium only). The lot-to-lot performance of HJyaluronidase Solution was consistent for enzyme activity and the removal of cumulus cells from freshly retrieved oocytes with in 2 minutes.

In addition to the mouse oocyte and embryo bioassays, Hyaluronidase Solution is also tested for enzyme activity using a standard enzymatic assay for Hyaluronidase. This permits verification of actual enzyme activity, in addition to the functional bioassay of cumulus dell removal and lack of toxicity.

Morover, stability studies have been conducted to determine the enzymatic activity of Irvine Scientific’s Hyaluronidase Solution after storage at several temperatures. The results is greater than 95% of the initial enzymatic activity (80IU/mL) remains after storage for 2 months at 6℃ (refrigerated) or 6 months frozen (-10℃).

The recommended storage temperature for this product is frozen (<-10℃) to assure a shelf life of at least 6 months. Repeated freeze/thaw cycles of Hyaluronidase Solution (no more than 3) did not adversely affect the enzymatic activity.

Hyaluronidase Solution

  • Available in kits of 5×1.0mL.
  • The shelf life is 6 months when stored sealed at the recommended temperature of (<-10℃).
  • Gentamicin sulfate is included as the antibiotic.

Features And Benefits

  • Convenient ready-to-use solution for denuding oocytes of cumulus cells prior to ICSI or other ART procedures. Refer to product insert for detailed usage instructions.
  • This solution contains HEPES buffered HTF medium to maintain proper pH during cumulus cell removal at ambient atmosphere conditions.
Reference Packaging
90101 5x1mL