Category: Washing

modified ham f

Modified Ham’s F-10 with Albumin

Description / Use

Modified Ham’s F-10 with Albumin is for in vitro sperm washing procedures. This buffering system does not require the use of a CO2 incubator.

  • (Fertil Steril 41:36, 1984; 46:914, 1986:48:93, 1987).
  • 21 mM HEPES buffer.
  • 4 mM Sodium Bicarbonate.
  • 1 mM Calcium Lactate.
  • 0.5 mM Magnesium Sulfate.
  • 5 mg/ml (0.5%) Human Albumin.
  • Without antibiotics.
  • Mouse embryo tested.
  • 12 x12 mL, 100 mL.
Reference Description
99109 100mL Mosidiws HAM’S F-10
99109 12x12mL Mosidiws HAM’S F-10
99168 100mL Mosidiws HAM’S F-10
99175 100mL Mosidiws HAM’S F-10

modified swm

Modified Sperm Washing Medium

Description / Use

Modified Sperm Washing Medium supports all steps of sperm handling prior to IUI, IVF, or other human ART procedures. Intended for in vitro sperm washing procedures which do not require CO2 incubation, Modified Sperm Washing Medium is a quality formulation that receives a complete laboratory evaluation with all results provided in a lot-specific Certificate of Analysis.

  • Formulation: HEPES-buffered HTF with bovine serum albumin (BSA).
  • Does not contain antibiotics.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Mouse Embryo Assay.
  • Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
9983 100ml Sperm Washing Media
9983 12x12ml Sperm Washing Media
9984 100ml Modified Sperm Washing
9984 12x12ml Modified Sperm Washing

mhm c with gentamicin

Multipurpose Handling Medium® -Complete (MHM®-C) with Gentamicin

Description / Use

Multipurpose Handling Medium® (MHM®) is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

  • Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
  • Support maintenance of cellular homeostasis with aid of glycine and taurine.
  • Reduce risk of toxicity using a dual buffer system.
  • Save time – no mixing or filtering, ready to use.
Reference Description
99166 100ml
99166 500ml

MHM With Gentamicin

Multipurpose Handling Medium® (MHM®) with Gentamicin

 

Description / Use

Multipurpose Handling Medium® (MHM®) with Gentamicin is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

  • Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
  • Support maintenance of cellular homeostasis with aid of glycine and taurine.
  • Reduce risk of toxicity using a dual buffer system.
  • Add preferred protein supplements as needed.
Reference Description
99166 100ml
99166 500ml

Sperm Washing Medium

Sperm Washing Medium

Description / Use

Sperm Washing Medium supports all steps of sperm preparation prior to fertilization, whether it is IUI, IVF, or other ART procedures. It is designed to maintain sperm quality on the tabletop without CO2 incubation. Sperm Washing Medium provides lot-to-lot consistency for sperm processing through its quality formulation, which is stringently tested by mouse embryo assay (MEA).

  • Formulation: HEPES-buffered HTF with human serum albumin.
  • Does not contain antibiotics.
  • CE Marked.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Mouse Embryo Assay.
  • Sterility by the current USP, CFR or Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
9983 100ml Sperm Washing Media
9983 12x12ml Sperm Washing Media
9984 100ml Modified Sperm Washing
9984 12x12ml Modified Sperm Washing