Category: Separation

Description / Use

ISolate Concentrate (100%) provides full control over the density gradient sperm separation process. Large volume packaging allows for economical development of custom gradients that meet your laboratory’s procedures and needs. As one of the most cited density gradient sperm separation media, ISolate Concentrate is the ideal product for customizable sperm separation.

• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM).
• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF.
• Quality tested by human sperm survival assay.
• CE Marked.

Quality Control Testing:

• Rabbit pyrogen test.
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
• Sperm Survival Assay.
• pH.
• Osmolality.
• Endotoxin by Limulus Amebocyte Lysate (LAL).

Description / Use

ISolate Stock is a 90% density gradient medium used for single layer sperm separation or as the lower layer of a customized gradient developed by the end user based on the semen quality. As one of the most cited density gradient sperm separation media, ISolate Stock is the ideal medium for laboratories that prefer single layer separation or more control over their sperm yields.

• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF.
• Quality tested by human sperm survival assay.
• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM).
• Supplied in large economical packages.
• CE Marked.

Quality Control Testing:

• Rabbit pyrogen test.
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test.
• Sperm Survival Assay.
• pH.
• Osmolality.

Description / Use

As one of the most cited density gradient sperm separation media, ISolate is the media of choice for laboratories worldwide to produce clean, robust samples with optimal recovery rates. It is supplied ready-to-use as a dual density gradient solution to process the wide variety of semen samples encountered in the laboratory. The kit, which consists of a 50% upper layer and 90% lower layer, support use with a two layer protocol for healthy samples or with a single layer protocol that enables separation of motile sperm from low quality semen samples. ISolate effectively removes cellular contaminants (dead sperm, white cells, and miscellaneous debris) to yield a sample that contains predominantly progressive, motile sperm, capable of fertilizing the oocyte for IUI, IVF and ICSI.

• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF.
• Highest quality raw materials.
• Quality tested by human sperm survival assay.
• 90% stock and 100% concentrate available for custom dilutions.
• CE Marked.

Quality Control Testing:

• Rabbit pyrogen test.
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test.
• Sperm Survival Assay.
• pH.
• Osmolality.
• Endotoxin by Limulus Amebocyte Lysate (LAL).