Category: Preservation / Thaw

refrigeration gentamicin

Refrigeration Medium – TYB with Gentamicin

Description / Use

Refrigeration Medium TYB with Gentamicin is ideal for short term sperm storage to preserve sperm for up to 4 days. With an easy one-step process of simply thawing the media and adding media at a 1:1 ratio with the sperm sample, it is quicker and easier than sperm cryopreservation. Refrigeration Medium TYB with Gentamicin contains Test-yolk buffer as the protecting agent, and the glycerol-free formulation avoids the potentially damaging effects from penetrating cryoprotectants. This product has been CE marked and FDA-cleared for short term sperm storage.

  • Contains
    – 20% egg yolk – from USDA certified Specific Pathogen Free (Virus Free) laying flocks, heat inactivated at 56℃ for 30 minutes
    – 10µg/mL Gentamicin Sulfate
    – Does not contain glycerol.
  • CE Mark.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Sperm Motility Recovery Assay.
  • Sterility by the current USP, CFR or Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refirigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media

freezing medium glycerol

Freezing Medium – TYB with Glycerol & Gentamicin

Description / Use

Freezing Medium TYB contains TEST-yolk buffer (TYB) to recover more motile sperm post-thaw than glycerol-only media (Hallak 2000, Nallella 2004). TEST-yolk buffer is a semen extender and cryobuffer that can be paired with glycerol to achieve high sperm motility and viability. The egg yolk is from USDA certified virus-free flocks and washed from the sperm after cryopreservation. Freezing Medium TYB is also the only CE-marked and FDA-approved sperm freezing media containing TYB and glycerol. As one of the most referenced sperm cryopreservation media worldwide, Freezing Medium TYB has established itself as the standard for performance.

  • 2 year shelf life.
  • Contains:
    – 20% egg yolk from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56&#8451 for 30 minutes.
    – 12% glycerol.
    – 10µg/mL gentamicin sulfate.
  • CE Marked.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Sperm Cryosurvival assay.
  • Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refirigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media