Description / Use

Embryo Freeze and Thaw Media kits consist of convenient, ready-to-use solutions designed for the cryopreservation and recovery of cleavage-stage embryos (zygote to 8-cell).

The composition of these solutions is based upon published standard propanediol and sucrose frezing (2 step) and thawing (3 step) protocols, which have proven to be the most clinically successful methods for cryopreservation and recovery of zygote to early cleavage stage human embryos, achieving similar implantation and pregnancy rates to that for transfers of fresh embryos.

Irvine Scientific has performed extensive efficacy testing on our Embryo Freeze and Thaw Media using mouse embryos and two different bioassay methods. Since standard MEA (exposure only) does not specifically emulate the intended application of these products; and as part of our ongoing efforts to enhance the sensitivity of our QC test methods, a specific Embryo Recovery Assay was developed and used to emulate the intended use of these products.

These solutions were also evaluated using the standard one-cell mouse embryo assay, typically employed as the accepted QC text method for commercially available formulas.

Independent IVF laboratories showed that the recovery of one-cell cryopreserved mouseembryos from Irvine Scientific’s Embryo Freeze and Thaw Media was equivalent to, or better than that achieved for their control cryopreservation media (in house prepared media using either propanediol / sucrose or DMSI methods) currently used in clinical practice for cryopreservation of human embryos.

Use of the embryo Recovery Assay (with one-cell mouse embryos) is a highly sensitive performance test for the intended use of Irvine Scientific’s Embryo Freeze and Thaw Media. High blastocyst development rates were obtained using the standard mouse embryo assay. Which confirm the lack of toxicity of these solutions. The use of these media can achieve up to 100% recovery of cryopreserved cleavage-stage embryos.

Embryo Freeze Media Kit

• Embryo Freeze Medium-F1,2x10mL
• Embryo Freeze Medium-F1,4x10mL

Embryo Thaw Media Kit

• Embryo Thaw Medium-T1,2x10mL
• Embryo Thaw Medium-T2,2x10mL
• Embryo Thaw Medium-T2,2x10mL

Features And Benefits

• Convenient ready-to-use kits consisting of two media for cryopreservation and three media for the thawing of embryos.
• Rapid protocol with just two steps to freeze and thee steps to thaw.
• Each solution contains HTF medium buffered with HEPES to maintain proper pH during cryopreservation and thawing procedures at ambient temperature and atmosphere.
• Gentamicin sulfate is included in each medium as an antibiotic.
• Store at 2-8℃.
• Shelf life of 6 months.