Vit Kit® – Freeze and Vit Kit® – Thaw
Description / Use
Irvine Scientific’s Vit Kit® – Freeze and Vit Kit® – Thaw consist of solutions optimized for vitrification and recovery of oocytes (MII stage)
and embryos (zygote to blastocyst stage).
Media Composition
The Composition of these solutions is based upon publish methods utilizing a combination of ethylene glycol (EG) and dimethylsulfoxide (DMSO) as the permeating cryoprotectants. The basal medium for these solutions consists of Modified M199 (containing 21mM HEPES buffer) supplemented with 20% DSS(HSA and Dextran, containing 10 mg/mL protein) and 35ug/mL gentamicin as the antibiotic.
| Solution | DMSO(v/v) | EG(v/v) | Sucrose |
|---|---|---|---|
| Equilibration Solution(ES) | 7.5% | 7.5% | 0M |
| Vitrification Solution(VS) | 15% | 15% | 0.5M |
| Thawing Solution(TS) | 0% | 0% | 1.0M |
| Dilution Solution(DS) | 0% | 0% | 0.5M |
| Washing Solution(WS) | 0% | 0% | 0 |
- Proven formulation that can be used for oocytes and all stages of embryos.
- Kits provide multiple applications and can be used on more than one patient.
- 8 weeks shelf-life after opening.
- The solutions are HEPES buffered to maintain pH during procedures performed at room temperature.
- Simple, straight forward protocols.
- Convenient configurations available with the CryoTip®, HSV straw, or with just the solutions.
- CryoTip® is an FDA approved closed freezing device.
Advantages of Irvine Scientific’s Vitrification system
| Reference | Discription | Packaging |
|---|---|---|
| 90133 Starter | ES 1x1mL | 10 Cryo Tips |
| 90133 Starter | VS 2x1mL | 10 Cryo Tips |
| 90137 SO | TS 4x1mL | |
| 90137 SO | DS 1x1mL | |
| 90137 SO | WS 1x2mL |
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