Description / Use

Hyaluronidase Solution is a convenient, ready-to-use solution designed for removing cumulus cells surrounding freshly retrieved oocytes prior to ICSI or other ART procedures.

This solution consists of 80 IU/mL of Hyaluronidase enzyme in a HEPES buffered HTF medium with therapeutic-grade human serum albumin (50mb/m:) and the antibiotic gentamicin sulfate (10µg/mL).

Irvine Scientific has performed extensive efficacy testing on Hyaluronidase Solution using freshly retrieved mouse oocytes and one-cell embryos.

The performance of Irvine Scientific’s Hyaluronidase Solution was equivalent to control hyaluronidase solutions, and no toxicity was observed to mouse oocytes and embryos. The development of one-cell embryos to blastocysts was equivalent after exposure to Hyaluronidase Solution as compared to non exposed controls (in growth medium only). The lot-to-lot performance of HJyaluronidase Solution was consistent for enzyme activity and the removal of cumulus cells from freshly retrieved oocytes with in 2 minutes.

In addition to the mouse oocyte and embryo bioassays, Hyaluronidase Solution is also tested for enzyme activity using a standard enzymatic assay for Hyaluronidase. This permits verification of actual enzyme activity, in addition to the functional bioassay of cumulus dell removal and lack of toxicity.

Morover, stability studies have been conducted to determine the enzymatic activity of Irvine Scientific’s Hyaluronidase Solution after storage at several temperatures. The results is greater than 95% of the initial enzymatic activity (80IU/mL) remains after storage for 2 months at 6℃ (refrigerated) or 6 months frozen (-10℃).

The recommended storage temperature for this product is frozen (<-10℃) to assure a shelf life of at least 6 months. Repeated freeze/thaw cycles of Hyaluronidase Solution (no more than 3) did not adversely affect the enzymatic activity.

Hyaluronidase Solution

• Available in kits of 5×1.0mL.
• The shelf life is 6 months when stored sealed at the recommended temperature of (<-10℃).
• Gentamicin sulfate is included as the antibiotic.

Features And Benefits

• Convenient ready-to-use solution for denuding oocytes of cumulus cells prior to ICSI or other ART procedures. Refer to product insert for detailed usage instructions.
• This solution contains HEPES buffered HTF medium to maintain proper pH during cumulus cell removal at ambient atmosphere conditions.