Description / Use

Freezing Medium TYB contains TEST-yolk buffer (TYB) to recover more motile sperm post-thaw than glycerol-only media (Hallak 2000, Nallella 2004). TEST-yolk buffer is a semen extender and cryobuffer that can be paired with glycerol to achieve high sperm motility and viability. The egg yolk is from USDA certified virus-free flocks and washed from the sperm after cryopreservation. Freezing Medium TYB is also the only CE-marked and FDA-approved sperm freezing media containing TYB and glycerol. As one of the most referenced sperm cryopreservation media worldwide, Freezing Medium TYB has established itself as the standard for performance.

• 2 year shelf life.
• Contains:
  – 20% egg yolk from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56&#8451 for 30 minutes.
  – 12% glycerol.
  – 10µg/mL gentamicin sulfate.
• CE Marked.

Quality Control Testing:

• Endotoxin by Limulus Amebocyte Lysate (LAL).
• Sperm Cryosurvival assay.
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
• pH.
• Osmolality.