Month: April 2018

ops

OPS

Description / Use

  • A single channel needle, triple-beveled, with a 5 mm grooved echogenic zone before the beveled tip.
  • A thin tubing connecting the needle to a bung. The latter is fitted onto a Falcon® type collecting tube.
  • A three-way valve (for the model concerned), to choose between aspiration or rinsing of the follicles.
  • A vacuum tube connecting the bung to the aspiration source.

The complete sets (needle + tubing) provide adapted storage conditions for the oocytes during the collection, particularly being able to maintain the vials at an appropriate temperature using heating systems. The resilient properties of polyurethane ensure flexibility without kinking for a regular flow rate, with no loss of suction. The specific quality of steel and polishing techniques improve the needle’s handling and sliding motion. The grooved circular markings provide echogenicity very close to the bevel.

Reference Description Packaging
1313101 OPS® ART of CCD® without valve Box of 10 units

vit kit thaw

Vit Kit®-Thaw

Description / Use

Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

4 Vials of TS (Thawing Solution, 4 x 2mL). Contains

  • 1 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of DS (Dilution Solution, 1 x 2mL). Contains

  • 0.5 M sucrose.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

1 Vial of WS (Washing Solution, 1 x 2mL). Contains

  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description
90137 TS, 4x1ml
90137 DS, 1x1ml
90137 WS, 1x2ml

vit kit freeze

Vit Kit®-Freeze

Description / Use

Vitrification Freeze Kit is for use in the vitrification of oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

2 Vials of ES (Equilibration Solution, 2 x 1 mL). Contains

  • 7.5% DMSO.
  • 7.5% ethylene glycol.
  • 20% DSS.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.

2 Vials of VS (Vitrification Solution, 2 x 1 mL). Contains

  • 15% DMSO.
  • 15% ethylene glycol.
  • 20% DSS.
  • 0.5 M sucrose.
  • Gentamicin.
  • In a M-199 HEPES Buffered Medium.
Reference Description Packaging
90133 ES, 1x1ml 10 Cryo Tips
90133 VS, 2x1ml 10 Cryo Tips

hsv straw red

HSV High Security Vitrification Straw – Red

Description / Use

High Security Vitrification (HSV) Red Straw (4 straws)

The only high security closed system for oocyte & embryo vitrification HSV straws provide the highest quality cryopreservation of biological samples.

Quality Control Testing:

  • Advanced Vitrification Techniques.
  • Vitrification and Warming of Blastocysts.
  • Pregnancy results following initiation of a blastocyst vitrification program.
  • Vitrification and warming of donor oocytes using a closed straw system.
Reference Description Packaging
025246 HSV High Security Vitrification kits – Red Pack of 4
025247 HSV High Security Vitrification kits – White Pack of 4
025248 HSV High Security Vitrification kits – Yellow Pack of 4
025249 HSV High Security Vitrification kits – Purple Pack of 4
025250 HSV High Security Vitrification kits – Blue Pack of 4
025251 HSV High Security Vitrification kits – Green Pack of 4

hyaluronidase solution

Hyaluronidase Solution

Description / Use

Hyaluronidase Solution is a convenient, ready-to-use solution designed for removing cumulus cells surrounding freshly retrieved oocytes prior to ICSI or other ART procedures.

This solution consists of 80 IU/mL of Hyaluronidase enzyme in a HEPES buffered HTF medium with therapeutic-grade human serum albumin (50mb/m:) and the antibiotic gentamicin sulfate (10µg/mL).

Irvine Scientific has performed extensive efficacy testing on Hyaluronidase Solution using freshly retrieved mouse oocytes and one-cell embryos.

The performance of Irvine Scientific’s Hyaluronidase Solution was equivalent to control hyaluronidase solutions, and no toxicity was observed to mouse oocytes and embryos. The development of one-cell embryos to blastocysts was equivalent after exposure to Hyaluronidase Solution as compared to non exposed controls (in growth medium only). The lot-to-lot performance of HJyaluronidase Solution was consistent for enzyme activity and the removal of cumulus cells from freshly retrieved oocytes with in 2 minutes.

In addition to the mouse oocyte and embryo bioassays, Hyaluronidase Solution is also tested for enzyme activity using a standard enzymatic assay for Hyaluronidase. This permits verification of actual enzyme activity, in addition to the functional bioassay of cumulus dell removal and lack of toxicity.

Morover, stability studies have been conducted to determine the enzymatic activity of Irvine Scientific’s Hyaluronidase Solution after storage at several temperatures. The results is greater than 95% of the initial enzymatic activity (80IU/mL) remains after storage for 2 months at 6℃ (refrigerated) or 6 months frozen (-10℃).

The recommended storage temperature for this product is frozen (<-10℃) to assure a shelf life of at least 6 months. Repeated freeze/thaw cycles of Hyaluronidase Solution (no more than 3) did not adversely affect the enzymatic activity.

Hyaluronidase Solution

  • Available in kits of 5×1.0mL.
  • The shelf life is 6 months when stored sealed at the recommended temperature of (<-10℃).
  • Gentamicin sulfate is included as the antibiotic.

Features And Benefits

  • Convenient ready-to-use solution for denuding oocytes of cumulus cells prior to ICSI or other ART procedures. Refer to product insert for detailed usage instructions.
  • This solution contains HEPES buffered HTF medium to maintain proper pH during cumulus cell removal at ambient atmosphere conditions.
Reference Packaging
90101 5x1mL

modified htf medium

Modified HTF Medium with Gentamicin – HEPES

Description / Use

Modified HTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos. mHTF is composed of a HEPES and sodium bicarbonate buffering system, making it ideal for use in ambient atmosphere conditions. It requires protein supplementation before use.

Quality Control Testing:

  • Store at 2-8℃.
  • 12 months from date of manufacture.
Reference Description
90166 100ml
90166 500ml

ceros

CEROS (Hamilton Throne)

Description / Use

If you replaced the internal optics of the IVOS II analyzer with a microscope and camera, what would you have? You would have a high performance analyzer at a lower price. You would have a CEROS II.

Save Space, Save Money

If you have limited bench space, a limited budget, or both – the CEROS is the answer for your computer assisted semen analysis needs. Providing the same sperm analysis accuracy and detail of the IVOS II, the CEROS II is a cost-effective and space-saving alternative. Since the CEROS II processor is a separate unit from the optical component, the computer can be placed under the bench and out of the way.

Components

  • A high speed computer running Windows 7
  • A negative phase contrast trinocular microscope (choice of Zeiss or Olympus)
  • A high resolution digital camera (analog camera is used for legacy Version 14 software)
  • A 24″ widescreen monitor
  • Mouse and keyboard

Ease of Use

The CEROS II hardware and software have been designed to provide users with a system that offers high-end performance while maintaining its ease of use. The intuitive Windows-based software promotes fast learning and quickly increases the confidence level of users. With a minimum investment of time, even users unfamiliar with computers will be performing fast, accurate and reliable sperm analyses. You don’t need to be a computer expert to analyze sperm with the CEROS II.

ivos hamilton throne

IVOS (Hamilton Throne)

Description / Use

Introducing the IVOS II Clinical – A Sleek New Look plus Powerful Features Designed to take CASA into the Future

Hamilton Thorne is pleased to announce the release of the IVOS II hardware platform. With this new design, we commit to the future development of the IVOS CASA system. The IVOS II Clinical may be used with the legacy Version 14 software (for USA) and the Human Motility II software.

  • Smoother, faster integrated stage featuring acceleration and deceleration. Thanks to a new stage drive motor, the IVOS stage now can move from maximum speed down to start speed in the blink of an eye.
  • Enhanced processing power is supplied by the latest generation of Intel technology – the “i Series.” With a minimum of 4 simultaneous processing threads, the IVOS is faster than ever.
  • A rearrangement of user controls and the addition of inputs on the IVOS front panel make for a better user experience:
    • On / Off switch added to the front panel
    • Stage LOAD button separated from the JOG buttons to avoid inadvertent stage loading/unloading.
    • Four high speed USB 2.0 ports for easy data transfer and connection to external devices
    • Blu-ray DVD burner/player – save up to 25 GB of data to Blu-ray Disc
  • Ruggedized Medical wide range AC/DC power supply
  • High definition, wide screen monitor
  • Wireless keyboard and mouse means less work area clutter
  • 1 Terabyte 6.0 GB/sec 7200 RPM hard drive
  • Dual Gigabit Ethernet ports
  • Dual digital monitor DVI connections plus display port connection
  • RAID drive option allows dual mirrored drive for reliability
  • Windows 8

Ease of Use

The IVOS II hardware and software have been designed to provide users with a system that offers high-end performance while maintaining its ease of use. The intuitive Windows-based software promotes fast learning and quickly increases the confidence level of users. Since the settings for the integrated optics are made through the software, mechanical adjustments are rarely required. With a minimum investment of time, even users unfamiliar with computers will be performing fast, accurate and reliable sperm analyses. You don’t need to be a computer expert to analyze sperm with the IVOS II.

Integrated Optics

As the only sperm analysis system with an internal optical system, the IVOS II offers distinct advantages to the sperm analysis laboratory. In contrast to the continuous illumination of a microscope, the IVOS uses illumination strobed at 1/1000 of a second to visualize sperm motion. This strobed illumination eliminates motion-related blurring along the length of the sperm head, resulting in precise sperm tracking. By adding an image capture rate of 60 frames per second, you get the highest level of accuracy available today for measuring sperm velocities and motion parameters. For added flexibility, the IVOS performs analyses under three types of internal, strobed illumination: phase contrast, bright field and multi-wavelength fluorescence.

Automated, Internal, Heated Specimen Stage

As part of the integrated optical system, the unique computer controlled specimen stage of the IVOS II provides precise control of temperature and position during analysis. The stage temperature, which may be set from ambient to 40°C, remains constant to within 0.5°C. For selection of analysis fields, the stage may be programmed for either manual or automatic field selection. For at-a-glance monitoring, the current stage temperature and position are continuously shown on screen as a real-time, digital display. The IVOS stage accommodates and automatically adjusts for commonly used analysis chambers, such as disposable fixed-depth slides, cannulas and Makler chambers. In addition, a user-defined chamber setting is available.

leja counting

Leja Counting

Description / Use

High quality disposable counting chambers for semen analysis. Due to their special characteristics, Leja slides will both save time and increase the level of accuracy and precision of semen analysis.

All slides are covered with a special coating to prevent air bubble formation and to prevent semen from sticking to the chamber surface. The resin and ink used are both non-toxic. The Leja slides have an excellent low limit of quantification and can handle high sperm concentrations. This has a direct clinical impact: you will be better prepared to determine the thresholds for the various modalities of Assisted Reproduction Technologies (waiting for natural conception, intrauterine insemination, in-vitro fertilization or intracytoplasmic sperm injection) and to decide which treatment is optimal for a couple.

The high level of accuracy and precision of the Leja chambers will allow your results to be compared with other laboratories’ scientific data concerning the relationship between semen parameters (concentration, motility, viscosity) and fertility.

Technical specifications

Slides with 2, 4 or 8 chambers

Depth: 10, 12, 20 or 100 microns

Reference Description Packaging
026853 10µ Leja slides – 4 wells 100 slides (4×25)
026854 12µ Leja slides – 2 lateral wells 100 slides (4×25)
026855 20µ Leja slides – 2 lateral wells 100 slides (4×25)
026354 20µ Leja slides – 2 wells 100 slides (4×25)
026355 20µ Leja slides – 4 wells 100 slides (4×25)
026856 20µ Leja slides – 8 wells 100 slides (4×25)
026357 100µ Leja slides – 2 wells high precision 100 slides (4×25)
026857 100µ Leja slides – 2 wells 100 slides (4×25)

straw cotton plug

CBS™ High Security Sperm Straws 0.3mL with Cotton Plug (100 Straws)

Description / Use

CBS™ High Security Sperm Straws 0.3mL with Cotton Plug — The CBS™ straw is a clear, flexible tube made of ionomeric resin for the storage and preservation of sperm. This straw is composed of two distinct parts separated by a sliding white cotton safety plug. The first part with a working volume of 0.3mL holds the sperm sample, while the second permits introduction of a colored identification tube and serves as a handle in liquid nitrogen.

Quality Control Testing:

  • L: 133 mm / Internal Ø: 2.25 mm.
  • Sterilized by irradiation.