andrology

Sperm Cryopreservation Media

Description / Use

Media containing Test-yolk buffer (TYB) has been shown to be effective in preserving sperm when refrigerated for short intervals of time, and for long term frozen storage with the addition of glycerol.

    sperm maintenance Medium with glycerol, but without Test-yolk buffer, is also designed for procedures involving the cryopreservation and storage of human sperm used for IUI, IVF and other assisted reproductive procedures.

    Features And Benefits

  • Formulated with Irvine Scientific’s WFI Grade Water.
  • Chemicals used in Irvine Scientific’s reproductive products meet UPS and ACS standards, where available.
  • All are membrane filtered and aseptically processed according to manufacturing procedures which have been validates to meet a sterility assurance level (SAL) of (10)-3

Refrigeration Medium – TYB with Gentamicin
for short term storage of human sperm at 2-5℃.

  • 20% egg yolk – from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56℃
  • 10µg/mL Gentamicin Sulfate.
  • Available in 20x5mL configuration.
  • Store below -10℃.
  • Shelf life: 2 years from date of manufacture.

Freezing Medium – TYB with Glycerol & Gentamicin
For cryoprotection when freezing human sperm.

  • 20% egg yolk – from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56℃ for 30 minutes.
  • 12% v/v Glycerol.
  • 10µg/mL Gentamicin Sulfate.
  • Available in 20x5mL and 100mL configuration.
  • Store below -10℃.
  • Shelf life: 2 years from date of manufacture.

Sperm Maintenance Medium
For cryoprotection when freezing human sperm.

  • 28% v/v Glycerol without egg yolk.
  • Available in 100mL configuration.
  • without antibiotics.
  • Store below -10℃.
  • 20mg/mL(2%) Human Serum Albumin.
  • Shelf life: 2 years from date of manufacture.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refrigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media

oil embryo culture

Oil For Embryo Culture

Description / Use

Oil for Embryo Culture, a sterile light mineral oil, is intended for use as an overlay when culturing in reduced volumes of media to prevent evaporation and to protect the media from changes in osmolality and pH.

Oil for Embryo Culture

  • Available in 100 mL bottles.
  • Oil for Embryo Culture has a shelf life of 2 years from date of manufacture, when Stored away from light, at 15-30℃.
  • Ready-to-use; Washing not required.

Features And Benefits

  • Each lot of Oil for Embryo Culture is tested for:
  • Endotoxin (USP<85>)
  • Bio-compatibility by MEA (one-cell)
  • Sterility (USP<71>and CFR Title 21 part 610.12)
  • Sterile filtered and aseptically processed, in a class 100 environment, which has been validated to meet a Sterility Assurance Level (SAL) of 10-3.

ivf media

Multipurpose Handling Medium

Description / Use

Buffered medium for oocyte retrieval, embryo transfer, micro-manipulation and gamete washing

Multipurpose Handling Medium Complete (MHM)

  • Available in 100ml and 500ml bottles.
  • MHM has a shelf life of 180 days from date of manufacture

Features And Benefits

    First IVF medium using a combination buffering system of HEPES and MOPS to optimize pH buffering and stability at room atmosphere:

  • Maintains pH across a broader range of temperatures.
  • Contains key amino acids, glycine and taurine, to help maintain cellular homeo stasis.
  • Formulated to promote optional cell growth by providing a safe and secure environment.

The first multipurpose dual buffering medium for use with gametes and embryos

Minimizing stress imposed upon gametes and embryos and maintaining homeostasis during in vitro manipulations are important for optimizing ART success. A key to this endeavor is the use of an appropriate handling medium utilized for cellular manipulations outside of the laboratory incubator.

MHM maintains an appropriate and stable pH during gamete and embryo manipulation at room atmosphere by employing the first combination buffering system containing safe and effective pH buffers, HEPES and MOPS By using these two proven pH buffers in specific ratios, pH buffering can be optimized for gametes and embryos and can lower amounts of each buffer utilized.

Reference Description Packaging
90166 MHM Complete 100 ml
90166 MHM Complete 500 ml

human tubal fluid

Human Tubal Fluid

Description / Use

HTF Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation through Day 3 of development

HTF Medium is a synthetic, defined solution for use as a culture media through Day 3 of human embryo development as well as the processing of gametes. HTF is bicarbonate-based and is designed for use in a CO2 incubator.

HTF Medium

  • Available as liquid medium in 100mL configuration. Custom configurations are available.
  • Has a shelf life of 64 days from the date of manufacture.
  • Requires protein supplementation.

Complete HTF Medium with SSS

  • Available as liquid medium in 2x20mL configuration.
  • Has a shelf life of 120 days from the date of manufacture.
  • Ready to use, already supplemented with protein (10% SSS) for a final total protein concentration of 6 mg/mL.
  • mHTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos.
    mHTF uses a buffering system composed of a 21 mM HEPES and 4 mM Sodium Bicarbonate combination. This buffering system does not require the use of a CO2 incubator.

mHTF Medium

  • Available as liquid medium in 100 mL configuration. Custom configurations are available.
  • Has a shelf life of 180 days from the date of manufacture.
  • Requires protein supplementation.
Reference Description Packaging
90125 HTF Media 100 ml
90125 HTF Media 500 ml
90126 mHTF 100 ml
90126 mHTF 500 ml

syms

SYSM

Manual sealer for CBS™ High Security Straws and vitrifcation straws

Reference Description Packaging
016399 SYMS sealer 1
009848 Spare kit for SYMS sealer 1

Cryo Bio System has developed a thermal pulse sealing device for impermeable sealing of CBS™ High Security straws in ionomeric resin. The sealing device is electronically operated by a push-button or foot pedal as desired.

Technical Specifications

Setup, maintenance and downtime time are approximately 5 minutes per day.
Potentially contaminated parts of the system can be cleaned with ethanol or non-corrosive decontamination fluids.

Dimensions (L x W x H) : 230 x 220 x 170 mm
Weight: 6 kg (13 lb)
Electrical requirements: 220V/50 Hz or 110V/60 Hz

nano digitcool freezer

Nano-Digitcool Controlled Rate Freezer, Compact And Efficient

The best compact freezer for day-to-day use in ART centers!

Description / Use

Controlled-rate freezing of all biological products (eg. plasma, all blood cells, human tissues, gametes). The stainless steel Digitcool freezer is fully programmable and easy to disinfect.

  • Latest addition to our Digitcool line
  • Vertical nitrogen flow with vaporization
  • PC or stand-alone operation
  • CryoBioSoft traceability software
  • Bags, straws and tubes freezing
  • Visual LED system for freezing steps

Technical Specifications

  • Dimensions : 722 x 475 x 472mm
  • Weight : 55 Kg (121 lb)
  • Dimensions of the chamber (Inside) : 238 x 178 x 200mm
  • Volume : 11.5 litres
  • Capacity : 33 straws 0.5 mL
  • Power (Heater) : 1200 w
  • Voltage / Frequency : 220 v / 50 Hz / 8 A
Reference Description Packaging
025680 Nano-Digitcool freezer 1
025433 Tubes rack for Nano-Digitcool 1
024572 Straws rack for Nano-Digitcool 1
025327 DF 200 bags rack for Nano-Digitcool 1

syms iii

SYMS III

Unique sealer for CBS High Security straws and tubes

Description / Use

The SYMS III thermally seals 0.3 mL, 0.5 mL and 2 mL CBS™ High Security straws as well as CBS™ High Security Vitrification straws and CBS™ High Security Tubes for top quality cryopreservation of biological samples.

Key Characteristics

  • A multilingual interface (French, English, Spanish and Chinese) + 4 optional languages
  • A color touch screen that can be used while wearing laboratory gloves
  • View of the machine cycles: preheating, ready status, sealing in progress, etc.
  • Specially adapted pre-programming for each CBS™ product’s sealing temperature and time
  • Two sealing methods: fully automatic detection or operator-controlled via the touch screen
  • Alarms and alert messages signal faults
  • Tracking display and maintenance modes display
  • Easy to clean and maintain

Technical Specifications

Dimensions (L x W x H) : 335 x 250 x 105 mm
Weight: 3 kg
Power Supply: 110 or 220 v / 60 or 50 Hz

Reference Description Packaging
025547 SYMS III sealer 1
026550 Sealing jaws set for SYMS III 1

syso

SYSO

New automatic equipment for CBS High Security Tubes opening

Description / Use

The SYSO opening machine is a worktop device for lab benches which can be used under laminar flow. It uses a hot wire to open CBS™ High Security Tubes.

Key Characteristics

  • The carrousel can hold up to 6 CBS™ High Security Tubes, opened individually or in succession.
  • Easy, intuitive touch screen
  • 8 languages
  • The wire is systematically sterilized by pyrolysis after each tube is opened.

Technical Specifications

L x W x H: 358 x 275 x 157 mm
Weight: 2.5 kg
Power Supply: 110 or 220 v / 60 or 50 Hz

Reference Description Packaging
024922 SYSO automatic equipment 1

pace

PACE

Semi-automatic filling and sealing system for CBS High Security Straws

Description / Use

From an open primary sample tube, PACE automatically fills and thermally seals both ends of CBS™ High Security 0.3 or 0.5 mL straws – which can be pre-identified. PACE is the ideal CBS™ machine for managing medium- or large-scale collection protocols. PACE is compatible with CBS™ High Security straws that are pre-identified with barcodes and/or alphanumerical codes, and those that are not yet identified. The system includes a filling and sealing unit and a control box.

General Characteristics

The throughput of straws is fully dependent on the protocol (e.g. number of straws filled per primary tube and the number of different biological products).
Setup, maintenance and shutdown: 15 minutes per day.

All potentially contaminated parts can be cleaned with ethanol or non-corrosive decontamination fluids.

The PACE system can be placed on the laboratory bench or under laminar flow with sterile straws and filling tubing and only needs power supply.

Technical Specifications

PACE – L x W x H: 380 x 300 x 370 mm – Weight: 25 kg

Control box – L x W x H: 410 x 365 x 155 mm – Weight: 14 kg

Power supply: 110 or 220 V / 60 or 50 Hz

A PACE can be configured for CBS™ 0.3 or 0.5 mL straws.

Reference Description Packaging
018451 PACE 220 V / 50 Hz 1
018450 PACE 110 V / 60 Hz 1
007454 Sterile CBS™ injection tubing (by 5 x 10) 50
018620 Sterile long blue CBS™ injection tubing (by 5 x 5) 25
007451 Sterile CBS™ aspiration tubing (by 5 x 10) 50

mapi

MAPI

Automatic filling, sealing and printing system for CBS High Security Straws

Description / Use

From an open primary sample tube, MAPI automatically fills CBS™ 0.3 or 0.5 mL straws, thermally seals both ends, prints bar codes and/or alphanumerical identification on the jacket and in-line validates the process by immediate bar code reading.

The MAPI system consists of the filling robot and its control box, a special ink-jet type printer and a PC with the MAPI pilot software. The automate is placed on a special laboratory bench at ergonomical height.

General Characteristics

The throughput of straws is fully dependent on the protocol (e.g. number of straws filled per primary tube and number of different biological products).
Setup, maintenance and shutdown: 15 minutes per day. All potentially contaminated parts can be cleaned with ethanol or non-corrosive decontamination fluids. The ink-jet printing system requires active evacuation of its solvent vapors.

MAPI Pilot Software

The MAPI pilot software is installed on the PC provided with the system to pilot the machine cycle of the MAPI system: distribution, filling, sealing, printing and re-reading of straws. The code to be printed on the straw can be either a bar code read from the primary tube, keyboard entered or programmed fixed text.

Technical Specifications

MAPI – L x W x H: 760 x 260 x 470 mm – Weight: 35 kg

Support table – L x W x H: 1200 x 600 x 900 mm – Weight with machine: 102 kg

Control box – L x W x H: 430 x 380 x 190 mm – Weight: 14 kg

Printer Domino control box – L x W x H: 650 x 450 x 375 mm – Weight: 20 kg

Reference Description Packaging
007218 MAPI automatic system 1
007454 Sterile CBS™ injection tubing (by 5 x 10) 50
018620 Sterile long blue CBS™ injection tubing (by 5 x 5) 25
007451 Sterile CBS™ aspiration tubing (by 5 x 10) 50