Month: April 2018

zona drilling pipettes

Zona Drilling Pipettes

Description / Use

  • SLM Zona Drilling Pipettes are used to apply acidic solution for creating a hole on the zona pellucida to enable assisted hatching and/or embryo biopsy.
  • There are two sizes available for selection:ID8-10µm and ID:10-12µm. These pipettes are designed with a slightly fire polished tip.
  • They are supplied with 0° -45° tip angle and a tip to elbow lenght of 0.55mm.
Catalogue No. Inner Diameter(µm) Tip Style Tip Angle Degree
SZD-10-xx 8-10 Flat 20°, 25°, 30°, 35°
SZD-12-xx 10-12 Flat 20°, 25°, 30°, 35°

holding pipettes

Holding Pipettes

Description / Use

  • SLM Holding Pipettes are used to hold an oocyte or embryo in position during ICSI and other micro-manipulation procedures.
  • There are three series and six sizes for selection. The classic series has an ID 15-20µm and OD in 90µm, 100µm,120µm and 130µm. The ‘S’ series has smaller ID(10-15µm). The ‘B’ series has an ID 25-30µm and OD in 120µm, 130µm and 1180µm. The ‘B’ series may by used for holding an oocyte with cumulus cells attached and for embryo biopsy.
  • All our holding pipettes are supplied with a tip angle of 0° to 45° and a tip elbow length of 0.65mm.
  • Packed in a box of 10.

Ordering Information

Catalogue No. Outer Diameter(µm) Inner Diameter(µm) Tip Angle Degree
SHP-70S-xx 70∓5 10-15 xx-0° to 45°
SHP-90-xx 90∓5 15-20 xx-0° to 45°
SHP-100-xx 100∓5 15-20 xx-0° to 45°
SHP-120-xx 120∓5 15-20 xx-0° to 45°
SHP-120B-xx 120∓5 25-30 xx-0° to 45°
SHP-130-xx 130∓5 15-20 xx-0° to 45°
SHP-130B-xx 130∓5 25-30 xx-0° to 45°
SHP-180B-xx 175∓5 25-30 xx-0° to 45°

icsi tools

ICSI Injection Pipettes

Description / Use

  • SLM ICSI Injection Pipettes are used to perform intracytoplasmic sperm injection (ICSI).
  • SLM offers three types of spiked and two types of non-spiked ICSI INJECTION PIPETTES.
  • SLM offers three sizes of ICSI Injection Pipette.
  • All our injection pipettes are supplied with an angle of 0° – 45° and a tip to elbow length of 0.55 mm and 1.0 mm.
Catalogue No. Size Tip Style Tip to Elbow Length Tip Angle Degree
SIC-45V-xx
SIC-45V-xxL
SIC-45W-xx
SIC-45W-xxL
SIC-45H-xx		 ID:4.0-4.5µm
OD:5-6µm		 Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°
SIC-45T-xx
SIC-45T-xxL
SIC-45N-xx
SIC-45N-xxL		 ID:4.0-4.5µm
OD:5-µm		 Non Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°
SIC-50V-xx
SIC-50V-xxL
SIC-50W-xx
SIC-50W-xxL
SIC-50H-xx		 ID:4.5-5.0µm
OD:6-7µm		 Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°
SIC-55V-xx
SIC-55V-xxL
SIC-55W-xx
SIC-55W-xxL
SIC-55H-xx		 ID:5.0-5.5µm
OD:6-7µm		 Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°

sysm iii b

SYMS III

Description / Use

The SYMS III sealer is a bench top unit that can be used under laminar rlow hood and is using the “thermal” procedure to weld CBS High Security 0.3mL, 0.5mL and 2mL straws, CBS High Security Tubes as well as the HSV High Security Vitrification Straw.

Technical Specifications

Setup, maintenance and downtime time are approximately 5 minutes per day.

Potentially contaminated parts of the system can be cleaned with ethanol or non-corrosive decontamination fluids.

L x W x H: 363 x 276 x 152mm
Weight: 6kg
Power Supply: 110 or 220V/60 or 50Hz

    Key Features

  • Available in 3 languages (French, English or Spanish).
  • Glove compatible touch screen interface.
  • Easy-to-use touch screen advises user of the machines actions:
    heating up, ready, welding process, etc.
  • Pre-set specific combination of seal temperature, time and jaw position for each CBS device.
  • Two sealing modes: fully automatic straw detection system or touch screen operator control.
  • Error management: alarm message on touch screen and audible signal.
  • Electronic touch screen monitors and advises on the maintenance needs of the machine.
  • Easy to clean and maintain.

    Instructions For Use

  • Place the CBS High Security straw of the CBS High Security tube holder;
  • Place the straw or tube on the holder and gently push forward;
  • Automatic mode: As soon as the sensor detects the straw or tube in the sealing position, the sealing process starts automatically.
  • Manual mode: the straw or tube is sealed when the operator presses the ‘WELD’ button on touch screen.
Reference Description Packaging
010287 CBS™ High Security sperm straw 0.3 mL, White cotton plug 4 cells of 5 straws
010288 CBS™ High Security sperm straw 0.3 mL, White cotton plug 4 cells of 5 straws
014651 CBS™ High Security sperm straw 0.5 mL, White cotton plug 4 cells of 5 straws
014650 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: white 5 cells of 20 straws
016584 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Red 5 cells of 20 straws
016611 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Yellow 5 cells of 20 straws
016612 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Green 5 cells of 20 straws
016613 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Blue 5 cells of 20 straws
016614 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Orange 5 cells of 20 straws
016615 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Gray 5 cells of 20 straws
007426 Recisperme with tube – Disposable sterile device for sperm collection  
015937 Isolating transport container for sperm tubes – Mix of colours  

storage liquid nitrogen

Storage In Liquid Nitrogen

Description / Use

Cryo Bio System carries a line of accessories for storage and management of HSV kits in liquid nitrogen.

    Thawing

    Thawing of the specimen should be done in a controlled environment, such as a laminar flow hood.

  • Prepare the thawing/dilution media
  • Identify the HSV straw
  • Transfer the straw from the storage container to a transport dewar filled with liquid nitrogen.
  • Lift the straw enough to expose the colored handling rod. Make sure the end with the sample remains immersed in the liquid nitrogen.
  • While holding the straw, use the opening device for HSV kit to section the straw.
  • Immediately (within 2 seconds), plunge the gutter into the first dilution media.

    Shelf-Life

  • Three years from date of manufacture.

embryo freeze thaw media

Embryo Freeze Thaw Media

Description / Use

Embryo Freeze and Thaw Media kits consist of convenient, ready-to-use solutions designed for the cryopreservation and recovery of cleavage-stage embryos (zygote to 8-cell).

The composition of these solutions is based upon published standard propanediol and sucrose frezing (2 step) and thawing (3 step) protocols, which have proven to be the most clinically successful methods for cryopreservation and recovery of zygote to early cleavage stage human embryos, achieving similar implantation and pregnancy rates to that for transfers of fresh embryos.

Irvine Scientific has performed extensive efficacy testing on our Embryo Freeze and Thaw Media using mouse embryos and two different bioassay methods. Since standard MEA (exposure only) does not specifically emulate the intended application of these products; and as part of our ongoing efforts to enhance the sensitivity of our QC test methods, a specific Embryo Recovery Assay was developed and used to emulate the intended use of these products.

These solutions were also evaluated using the standard one-cell mouse embryo assay, typically employed as the accepted QC text method for commercially available formulas.

Independent IVF laboratories showed that the recovery of one-cell cryopreserved mouseembryos from Irvine Scientific’s Embryo Freeze and Thaw Media was equivalent to, or better than that achieved for their control cryopreservation media (in house prepared media using either propanediol / sucrose or DMSI methods) currently used in clinical practice for cryopreservation of human embryos.

Use of the embryo Recovery Assay (with one-cell mouse embryos) is a highly sensitive performance test for the intended use of Irvine Scientific’s Embryo Freeze and Thaw Media. High blastocyst development rates were obtained using the standard mouse embryo assay. Which confirm the lack of toxicity of these solutions. The use of these media can achieve up to 100% recovery of cryopreserved cleavage-stage embryos.

    Embryo Freeze Media Kit

  • Embryo Freeze Medium-F1,2x10mL
  • Embryo Freeze Medium-F1,4x10mL

    Embryo Thaw Media Kit

  • Embryo Thaw Medium-T1,2x10mL
  • Embryo Thaw Medium-T2,2x10mL
  • Embryo Thaw Medium-T2,2x10mL

    Features And Benefits

  • Convenient ready-to-use kits consisting of two media for cryopreservation and three media for the thawing of embryos.
  • Rapid protocol with just two steps to freeze and thee steps to thaw.
  • Each solution contains HTF medium buffered with HEPES to maintain proper pH during cryopreservation and thawing procedures at ambient temperature and atmosphere.
  • Gentamicin sulfate is included in each medium as an antibiotic.
  • Store at 2-8℃.
  • Shelf life of 6 months.
Reference Packaging
90116 6x10mL
90124 6x10mL

vitrification freeze

Vit Kit® – Freeze and Vit Kit® – Thaw

Description / Use

Irvine Scientific’s Vit Kit® – Freeze and Vit Kit® – Thaw consist of solutions optimized for vitrification and recovery of oocytes (MII stage)
and embryos (zygote to blastocyst stage).

Media Composition

The Composition of these solutions is based upon publish methods utilizing a combination of ethylene glycol (EG) and dimethylsulfoxide (DMSO) as the permeating cryoprotectants. The basal medium for these solutions consists of Modified M199 (containing 21mM HEPES buffer) supplemented with 20% DSS(HSA and Dextran, containing 10 mg/mL protein) and 35ug/mL gentamicin as the antibiotic.

Solution DMSO(v/v) EG(v/v) Sucrose
Equilibration Solution(ES) 7.5% 7.5% 0M
Vitrification Solution(VS) 15% 15% 0.5M
Thawing Solution(TS) 0% 0% 1.0M
Dilution Solution(DS) 0% 0% 0.5M
Washing Solution(WS) 0% 0% 0

    Advantages of Irvine Scientific’s Vitrification system

  • Proven formulation that can be used for oocytes and all stages of embryos.
  • Kits provide multiple applications and can be used on more than one patient.
  • 8 weeks shelf-life after opening.
  • The solutions are HEPES buffered to maintain pH during procedures performed at room temperature.
  • Simple, straight forward protocols.
  • Convenient configurations available with the CryoTip®, HSV straw, or with just the solutions.
  • CryoTip® is an FDA approved closed freezing device.
Reference Discription Packaging
90133 Starter ES 1x1mL 10 Cryo Tips
90133 Starter VS 2x1mL 10 Cryo Tips
90137 SO TS 4x1mL  
90137 SO DS 1x1mL  
90137 SO WS 1x2mL  

icsi media

Polyvinylpyrrolidone (PVP) With HSA

Description / Use

Ployvinylpyrrolidone (PVP-K90) is a synthetic copolymer with a molecular weight of 360,000 Daltons. PVP is intended for use in ICSI procedures involving immobilization of sperm.

PVP with HSA solution is a ready-to-use PVP with HSA (5mg/mL), reconstituted in an isotonic HEPES buffered HTF medium.

7% PVP With HSA Solution

  • Recommended for use with low quality or low motility sperm specimens.
  • Available in kits of 5×0.5mL.
  • Has a shelf-life of 6 months from the date of manufacture when stored at the recommended temperature of 2℃ to 8℃

10% PVP With HSA Solution

  • Recommended for use with highly motile sperm specimens.
  • Available in kits of 5×0.5mL.
  • Has a shelf-life of 6 months from the date of manufacture when stored at the recommended temperature of 2℃ to 8℃.

    • Lyophilized PVP* is composed of USP grade PVP which is dissolved in Water for Injection (WFI) grade water and then membrane (SAL10) filtered and lyophilized.

10% Lyophilized PVP

  • Available in kits of 10x1mL.
  • Requires aseptic reconstitution with an appropriate medium (such as sperm washing medium) prior to use.
  • Has a shelf life of 2 years from the date of manufacture, once reconstitute lyophilized PVP mau be stored for up to 3 weeks when stored at the recommenced temperature of 2℃p to 8℃.
  • Does not contain protein supplement.
Reference Packaging
90121 5×0.5mL (7%)
90123 5×0.5mL (10%)

isolated sperm

Isolate® Sperm Separation

Description / Use

Isolate® is a superior medium for separation of the motile fraction of sperm from its surrounding seminal plasma by density centrifugation.

As a two-layer gradient system, ISolate effectively removes cellular contaminants-dead sperm, white cells, and miscellaneous debris-yielding a sample that contains predominately motile sperm.

ISolate is a sterile, colloidal suspension of silica particles stabilized with covalently bound hydrophilic silane is a HEPES-buffered HTF. This buffered system maintains a physiological pH at ambient conditions (do not use this product in a CO2 incubator unless tightly capped).

a fresh or cryopreserved semen sample is overlaid on 1.5 to 2.0mL aliquots of the two-layer gradient system in a conical centrifuge tube. The prep tube is then centrifuged at a low speed, ~300xg for 20 minutes. This action causes seperation of the seminal components. Dead sperm and debris are found in the upper portion of the tube while a fraction of highly motile, morphologically normal cells will form a pellet in the bottom.

Irvine Scientific has conducted extensive performance and safety testing on ISolate. These studies indicate that it is comparable or superior to the standard separation techniques utilizing Percoll® and superior in performance to standard swing-up procedures.

ISolate

    50% upper layer and 90% lower layer.

  • Ready-to-use, no dilutions necessary.
  • Three available configurations to chose form
      12x6mL vials ~18 Isolations
      2x50mL vials ~25 Isolations
      2x100mL vials ~50 Isolations

ISolate Stock Solution

    90% density gradient medium “ISotonic” stock solution allows end user to make their own working dilutions where two layer ISolations are preferred.

  • Available in 100mL bottles.

ISolate Concentrate

  • 100% colloidal silica concentrate.
  • Available in 100mL bottles.

    • Larger volume custom fill sizes available.

Features and Benefits

  • Rabbit pyrogen tested.
  • Sperm survival assay tested.
  • Certificate of Analysis provided for each lot manufactured.
  • Shelf-Life is two years when stored at 2℃ to 8℃.
Reference Packaging
99264 12x6mL
99264 2x100mL
99264 2x50mL
99275 100mL(90%)
99306 100mL(100%)

sperm washing media

Sperm Washing Media

Description / Use

Sperm Washing Media are intended for washing sperm prior to IUI, IVF and other human ART procedures.

Features And Benefits

  • Formulated with Irvine Scientific’s WFI grade Water.
  • Chemicals used in Irvine Scientific’s reproductive products meet USP and ACS standards, where available.
  • all are mebrane filtered and aseptically processed according to manufacturing procedures which have been validated to meet a sterility assurance level (SAL) of (10)-3.

Sperm Washing Medium
for in vitro sperm washing procedures which do not require CO2 incubation.

  • Based on Modified HTF Medium.
  • 21mM HEPES buffer.
  • 4mM Sodium Bicarbonate.
  • 5mg/mL(0.5%) Human Serum Albumin.
  • Without antibiotics.
  • Mouse embryo tested.
  • Shelf life: 2 years from date of manufacture.
  • Store at 2-8℃.
  • Available in 12x12mL $100mL configurations.

Modified Sperm Washing Medium
For in vitro sperm washing procedures which do not require CO2 incubation.

  • Based on Modified HTF Medium.
  • 21mM HEPES buffer.
  • 4mM Sodium Bicarbonate.
  • 5mg/mL(0.5%) Bovine Serum Albumin.
  • Without antibiotics.
  • Mouse embryo tested.
  • Shelf life: 2 years from date of manufacture.
  • Store at 2-8℃.
  • Available in 12x12mL & 100mL configurations.

Modified Ham’s F-10 with Albumin
for in vitro sperm washing procedures which do not require CO2 incubation.

  • 21mM HEPES buffer.
  • 4mM Sodium Bicarbonate.
  • 1mM Calcium Lactate.
  • 0.5mM Magnesium Sulfate.
  • 5mg/mL(0.5%) Bovine Serum Albumin.
  • Without antibiotics.
  • Mouse embryo tested.
  • Shelf life: 2 years from date of manufacture.
  • Store at 2-8℃.
  • Available in 12x12mL & 100mL configurations.
Reference Description
9983 100mL Sperm Washing Media
9983 12x12mL Sperm Washing Media
9984 100mL Modified Sperm Washing
9984 12x12mL Modified Sperm Washing