Month: April 2018

hse sperm straw

High Security Embryo/Sperm Straws

Description / Use

High Security Embryo/Sperm Straws are designed for the cryopreservation of embryos or sperm. Manufactured of biocompatible materials, the straws have a three-part plug and contain tamper-proof colored identification rods to guarantee sample traceability.

Quality Control Testing:

  • Store at 15-30℃.
  • L: 133 mm / Internal Ø: 2.25 mm.
  • Sterilized by irradiation.
  • 3 years from of date of manufacture.

rigid straw 91

Rigid Embryo Straw, 91 mm

Description / Use

Rigid Embryo Straws are used for freezing and storage of human embryos.

PETG embryo straws are flexible or rigid clear tubes made of copolymer. The PETG embryo straws are closed at one end by 2 woven plugs enclosing a dose of powder that polymerizes when in contact with liquid.

Reference Description Packaging
006433 Sterile clear rigid embryo straw 0.15 mL – 91 mm – 4 cells x 5 straws 20 (4 x 5)
006578 Sterile clear rigid embryo straw 0.25 mL – 133 mm – 4 cells x 5 straws 20 (4 x 5)

refrigeration gentamicin

Refrigeration Medium – TYB with Gentamicin

Description / Use

Refrigeration Medium TYB with Gentamicin is ideal for short term sperm storage to preserve sperm for up to 4 days. With an easy one-step process of simply thawing the media and adding media at a 1:1 ratio with the sperm sample, it is quicker and easier than sperm cryopreservation. Refrigeration Medium TYB with Gentamicin contains Test-yolk buffer as the protecting agent, and the glycerol-free formulation avoids the potentially damaging effects from penetrating cryoprotectants. This product has been CE marked and FDA-cleared for short term sperm storage.

  • Contains
    – 20% egg yolk – from USDA certified Specific Pathogen Free (Virus Free) laying flocks, heat inactivated at 56℃ for 30 minutes
    – 10µg/mL Gentamicin Sulfate
    – Does not contain glycerol.
  • CE Mark.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Sperm Motility Recovery Assay.
  • Sterility by the current USP, CFR or Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refirigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media

freezing medium glycerol

Freezing Medium – TYB with Glycerol & Gentamicin

Description / Use

Freezing Medium TYB contains TEST-yolk buffer (TYB) to recover more motile sperm post-thaw than glycerol-only media (Hallak 2000, Nallella 2004). TEST-yolk buffer is a semen extender and cryobuffer that can be paired with glycerol to achieve high sperm motility and viability. The egg yolk is from USDA certified virus-free flocks and washed from the sperm after cryopreservation. Freezing Medium TYB is also the only CE-marked and FDA-approved sperm freezing media containing TYB and glycerol. As one of the most referenced sperm cryopreservation media worldwide, Freezing Medium TYB has established itself as the standard for performance.

  • 2 year shelf life.
  • Contains:
    – 20% egg yolk from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56&#8451 for 30 minutes.
    – 12% glycerol.
    – 10µg/mL gentamicin sulfate.
  • CE Marked.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Sperm Cryosurvival assay.
  • Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
90128 Freezing Media TYB with Glycerol & Gentamicin
90129 Refirigeration Media TYB with Gentamicin
99176 Sperm Maintenance Media

modified ham f

Modified Ham’s F-10 with Albumin

Description / Use

Modified Ham’s F-10 with Albumin is for in vitro sperm washing procedures. This buffering system does not require the use of a CO2 incubator.

  • (Fertil Steril 41:36, 1984; 46:914, 1986:48:93, 1987).
  • 21 mM HEPES buffer.
  • 4 mM Sodium Bicarbonate.
  • 1 mM Calcium Lactate.
  • 0.5 mM Magnesium Sulfate.
  • 5 mg/ml (0.5%) Human Albumin.
  • Without antibiotics.
  • Mouse embryo tested.
  • 12 x12 mL, 100 mL.
Reference Description
99109 100mL Mosidiws HAM’S F-10
99109 12x12mL Mosidiws HAM’S F-10
99168 100mL Mosidiws HAM’S F-10
99175 100mL Mosidiws HAM’S F-10

modified swm

Modified Sperm Washing Medium

Description / Use

Modified Sperm Washing Medium supports all steps of sperm handling prior to IUI, IVF, or other human ART procedures. Intended for in vitro sperm washing procedures which do not require CO2 incubation, Modified Sperm Washing Medium is a quality formulation that receives a complete laboratory evaluation with all results provided in a lot-specific Certificate of Analysis.

  • Formulation: HEPES-buffered HTF with bovine serum albumin (BSA).
  • Does not contain antibiotics.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Mouse Embryo Assay.
  • Sterility by the current USP, CFR and Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
9983 100ml Sperm Washing Media
9983 12x12ml Sperm Washing Media
9984 100ml Modified Sperm Washing
9984 12x12ml Modified Sperm Washing

mhm c with gentamicin

Multipurpose Handling Medium® -Complete (MHM®-C) with Gentamicin

Description / Use

Multipurpose Handling Medium® (MHM®) is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

  • Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
  • Support maintenance of cellular homeostasis with aid of glycine and taurine.
  • Reduce risk of toxicity using a dual buffer system.
  • Save time – no mixing or filtering, ready to use.
Reference Description
99166 100ml
99166 500ml

MHM With Gentamicin

Multipurpose Handling Medium® (MHM®) with Gentamicin

 

Description / Use

Multipurpose Handling Medium® (MHM®) with Gentamicin is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

  • Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range.
  • Support maintenance of cellular homeostasis with aid of glycine and taurine.
  • Reduce risk of toxicity using a dual buffer system.
  • Add preferred protein supplements as needed.
Reference Description
99166 100ml
99166 500ml

Sperm Washing Medium

Sperm Washing Medium

Description / Use

Sperm Washing Medium supports all steps of sperm preparation prior to fertilization, whether it is IUI, IVF, or other ART procedures. It is designed to maintain sperm quality on the tabletop without CO2 incubation. Sperm Washing Medium provides lot-to-lot consistency for sperm processing through its quality formulation, which is stringently tested by mouse embryo assay (MEA).

  • Formulation: HEPES-buffered HTF with human serum albumin.
  • Does not contain antibiotics.
  • CE Marked.

Quality Control Testing:

  • Endotoxin by Limulus Amebocyte Lysate (LAL).
  • Mouse Embryo Assay.
  • Sterility by the current USP, CFR or Ph. Eur. Sterility Test.
  • pH.
  • Osmolality.
Reference Description
9983 100ml Sperm Washing Media
9983 12x12ml Sperm Washing Media
9984 100ml Modified Sperm Washing
9984 12x12ml Modified Sperm Washing